【摘要】 目的检测肝脏X受体(Liver X receptor alpha,LXRα)在HBV阳性肝硬化组织及原发性肝癌中的表达情况及临床意义。方法收集20例揭阳市人民医院及汕头大学医学院第一附属医院手术切除的HBV阳性肝硬化原发性肝癌组织标本,应用免疫组化染色检测LXRα表达。Huh7细胞中分别过表达小鼠mLXRα,及共过表达HBx(HBV X protein)和mLXRα,并检测mLXRα及HBx对内源性LXRα信号通路下游靶基因SREBP-1(Sterol regulatory element-binding protein 1)的影响;双荧光素酶报告系统检测人肝癌Huh7细胞中HBx对LXRα转录活性的影响。结果 LXRα在肝硬化组织中阳性表达率为95%,高于癌组织的25%。HBx上调SREBP-1的表达可能与HBx调控LXRα的转录活性有关。结论 HBx通过调节癌前高表达的LXRα的转录活性,并影响其信号通路可能与HCC发生相关。更多还原

【Abstract】 Objective To detect the expression of liver X receptor alpha(LXRα) in HBV infection associated liver cirrhosis and hepatocellular carcinoma tissues and explore its clinical significance. Methods The expression of LXRα in 20 cases of HBV infection-associated liver cirrhosis and hepatocellular carcinoma specimens from Jieyang People’s Hospital and the First Affiliated Hospital of Medical College, Shantou University was detected by immunohistochemical staining. Endogenous SREBP-1, a typical LXRα target gene, was detected by real-time PCR after over-expressing mice mLXRα, HBx and mLXRα in Huh7 cells. The transcriptional regulation of LXRα signaling by HBx was evaluated by dual luciferase reporter system. Results The positive expression rate of LXRα was 95% in cirrhosis, which was higher than that in HCC tissue with only 25%. The up-regulation of SREBP-1 expression by HBx may be related with the transcriptional activation of LXRα by HBx. Conclusion HBx maybe associated with the development of HCC by regulation of the transcriptional activation of LXRa, which was higher in cirrhosis, and activating the signal pathway更多还原