【摘要】 目的探讨结膜印迹细胞学检查(CIC)在干眼症诊断中的作用。方法选择2011年1月至2013年6在我院就诊的干眼症患者180例为观察组,按干眼症的分级,轻度65例,中度58例,重度57例;另选择180例健康者作为对照组。所有研究对象均进行泪液分泌实验(S-I-T)、泪膜破裂时间(BUT)、荧光素染色(FLS)检查;同时重点进行CIC检查。结果 S-I-T、BUT、FLS检查指标结果,观察组与对照组差异均有统计学意义(P<0.01)。观察组杯状细胞数量明显少于对照组(P<0.01);观察组轻、中、重度患者的杯状细胞数均少于对照组(P<0.05或P<0.01)。对照组CIC分级主要集中于0级和1级;干眼症患者则多集中于1、2、3级;其中轻、中度多集中于1、2级,重度主要为3级。观察组与对照组CIC分级有明显不同,差异均有统计学意义(P<0.05)。观察组轻、中、重度患者CIC分级分别与对照组比较差异均有统计学意义(P<0.05或P<0.01)。结论 CIC检查能够反映泪膜黏液层的病理变化情况,可用于区分正常人与干眼症患者并可了解干眼的病程变化。更多还原

【Abstract】 Objective To evaluate the applicability of Conjunctival impression cytology(CIC) in the diagnosis of dry eye. Methods A total of 180 patients with dry eye in our hospital from Jan. 2011 to Jun. 2013 were assigned into observation group and another 180 healthy physical examinees were selected as control group. Both groups were tested by CIC in addition to Schirmer Test(S-I-T), Tear Break-Up Time(BUT), and Corneal Fluorescein Staining(FLS). And then the results were compared. Results There was a significantly difference in indexes of S-I-T, BUT, FLS and CIC between the observation group and the control group(P<0.01). The number of goblet cells in the observation group was less than that in the control group(P<0.01). Furthermore, the number of goblet cells of the mild, moderate and severe patients in the observation group were all less than those in the control group(P<0.05 or P<0.01). The controls were mostly graded to 0 or 1 revealed by CIC, while patients in the observation group were graded as 1, 2 or 3 with the same method. In the observation group, mild and moderate patients were revealed to be grade 1 or grade 2, while severe patients were grade 3. There was a significant difference in CIC grade between the observation group and the control group(P<0.01). The CIC Grades of mild, moderate, and severe patients in the observation group were all significantly different from those in the control group(P<0.05 or P<0.01). Conclusion CIC grading and goblet cell density can be used to distinguish dry eye patients and track the progression of dry eye as well.更多还原