【摘要】 从喇叭菌子实体中分离纯化得到喇叭菌多糖CCPⅠ,通过高效凝胶渗透色谱法和气相色谱法测定其相对分子质量和单糖组成,分析了它的红外光谱特征和核磁共振图谱。研究表明,喇叭菌多糖CCPⅠ的重均相对分子质量为16270 Da,具有典型的多糖红外吸收,是一种含有α和β型吡喃糖苷键的杂多糖,由岩藻糖、甘露糖、葡萄糖和半乳糖组成,摩尔比为1:2.11:3.13:6.45。糖链中C-6大部分被取代,C-2、C-3或C-4部分被取代。采用体外抗补体活性测定方法和四甲基偶氮唑盐微量酶反应比色法评价了喇叭菌多糖CCPⅠ的抗补体活性和抗肿瘤活性,发现当CCPⅠ浓度为4 mg/mL时,抗补体活性达到70.44%,对人肝癌细胞Hep G2和人体乳腺癌细胞MCF-7的增殖均有明显抑制作用且呈剂量依赖性,当CCPⅠ浓度为1000μg/mL时,对Hep G2和MCF-7抑制率分别达到60.06%和55.80%。更多还原

【Abstract】 One polysaccharide, named CCPⅠ, was isolated and purified from the sporophore of Craterellus cornucopioides. The relative molecular mass and monosaccharide composition of CCPⅠ were determined by using high-performance gel permeation chromatography and gas chromatography, and its spectra of IR and NMR were also investigated. The weight-average relative molecular mass of CCPⅠ was 16270 Da. It possessed typical infrared absorptions of polysaccharides and showed α-and β-configuration pyranoside. In addition, it was determined as a kind of heteropolysaccharide containing fucose, mannose, glucose and galactose in a molar ratio of 1:2.11:3.13:6.45. Most of glycan C-6 were substituted, and part of C-2, C-3 or C-4 was replaced. Moreover, its anti-complement activity in vitro and antitumor activity by MTT were evaluated, which indicated that the anti-complement activity reached 70.44% when the concentration of CCP was 4 mg/mLⅠ and it remarkably inhibited the growth of human liver cancer cell line Hep G2 and human breast cancer cell line MCF-7 in a dose-dependent manner. When the concentration of CCP was 1000 Ⅰμg/mL,the inhibition ratios of Hep G2 and MCF-7 were 60.06%and 55.80%,respectively.更多还原