【摘要】 十二烷基肌氨酸钠与天然细胞色素c通过自组装方式构建了一种新型纳米胶团结构人工过氧化物酶(Artificial Peroxidase,AP)。采用紫外可见光分光光度计对AP酶促反应过程进行研究。表明AP在较宽的pH范围具有活性,并在pH 5.0,100 mmol/L磷酸盐缓冲液中达到最大。该AP的米氏常数Km、催化速率kcat以及催化效率分别为1.70μmol/L、0.11 s-1、0.065μM-1s-1,催化效率为天然辣根过氧化物酶的90.2%。更多还原

【Abstract】 A nanostructured artificial peroxidase( AP) was constructed self-assembly using cytochrome c and sodium N-lauroyl sarcosine nano miceller. UV-vis spectroscopic study showed that the AP had peroxidase activity in a wide pH range with maximum peroxidase activity at pH 5. 0. The Michaelis-Menten( Km),catalytic rate( kcat) and catalytic efficiency were obtained to be 1. 70 μmol /L,0. 11 s-1,0.065 μM-1s-1,respectively. The catalytic efficiency of the AP was 90. 2% as efficient as the native horseradish peroxidase( HRP).更多还原