【摘要】 目的 Fascin-1是一种与F-肌动蛋白结合的55kDa细胞骨架蛋白,并参与细胞膜突起的形成及细胞的迁移。目前已知在多种肿瘤细胞中呈现高表达。本研究的目的探讨fascin-1蛋白在骨巨细胞瘤中的表达及其与相关临床参数之间的关系。方法 65例骨巨细胞瘤患者均来自唐山市第二医院,其中男30例,女35例;平均年龄35(9~58)岁。患者平均随访时间51.5(12.5~80.0)个月。应用免疫组化检测65例骨巨细胞瘤组织中fascin-1蛋白的表达情况,并与临床病理数据进行统计学分析。结果 65例骨巨细胞瘤标本中有40例表现为阳性表达,阳性率为66.7%,主要表现在细胞浆中。Fascin-1表达与各种反映肿瘤预后的指标进行相关性研究结果表明,在36例良性骨巨细胞瘤患者中有20例呈阳性表达,占55.6%,而在27例侵袭性患者中有19例呈阳性表达,占70.3%;在2例恶变及转移患者均呈阳性表达;对比预后良好的患者,16例复发患者中10例呈阳性表达,占62.5%。统计表明fascin-1蛋白的表达与肿瘤分类及预后相关(P<0.05),而与性别、年龄、肿瘤部位及大小无明显相关(P>0.05)。结论 Fascin-1蛋白在骨巨细胞瘤组织中呈现高表达,可能在骨巨细胞瘤的复发及恶变过程中发挥重要作用,并可能成为我们治疗骨巨细胞瘤的一个新的靶点。更多还原

【Abstract】 Objective Fascin-1 is the 55 kilodalton(kDa) F-actin-binding cytoskeletal protein,which takes part in the formation of cellular membrane protrusions and cell migration.Fascin-1 is upregulated in several types of carcinomas.To investigate the relationship between the fascin-1 expressions in giant cell tumors of bone and the related clinical parameters.Methods A total of 65 patients with giant cell tumors of bone were adopted by the second Hospital of Tangshan,including 30 males and 35 females.Their average age was 35 years old(range:9-58 years).The average follow-up period was 51.5 months(range:12.5-80.0 months).Immunochemistry with digital image analysis was used to determine the fascin-1 protein expressions in histologic,sections from the 65 patients.The fascin-1 expressions of65 tissue samples were detected by immunochemistry.The fascin-1 expressions with the clinicopathologic features of giant cell tumors of bone were assessed.Results The fascin-1 expressions were positive in 40 of the 65 patients with giant cell tumors of bone,and the positive rate was 66.7%.Cytoplasm was the main location.A correlation study was performed between the fascin-1 expressions and various indexes reflecting the tumor prognosis.The results showed positive expressions were noticed in 20 of 36 patients with benign giant cell tumors of bone(55.6%) and in 19 out of 27 patients with invasive tumors(70.3%).The fascin-1 expressions were positive in 2 patients with malignant and metastatic tumors.Patients with good prognosis were observed.The positive expressions were found in 10 out of16 patients with recurrence,accounting for 62.5%.The data above demonstrated that the fascin-1 expressions were significantly correlated with the classification and prognosis of tumors(P<0.05),but not with the gender,age,tumor location or tumor size(P>0.05).Conclusions Fascin-1 is upregulated in giant cell tumors of bone,which may play an important role in their recurrence and malignant transformation.So fascin-1 could be a new target in the treatment of giant cell tumors of bone.更多还原