【摘要】 利用Red同源重组技术敲除大肠杆菌BL21中relA基因,获得△relA突变株。进一步研究表明在LB复合培养中relA基因对大肠杆菌的生长几乎没有明显影响,但是对其重组蛋白的合成有着较大的影响:降低了25%。结果表明,relA基因在大肠杆菌表达重组蛋白方面有着较重要的作用。更多还原

【Abstract】 The Red recombination system was used to knock out the relA gene in E.coli BL21 and then the △relA mutant was obtained.Further study was conducted and results showed that the relA gene had no effect on its growth while significant difference in terms of synthesis of recombinant protein was found between these two strains.It reduced by 25%compared with the original strain.Results showed that the relA gene may play an important role in synthesis of recombinant protein in E.coli BL21.更多还原