【摘要】 目的优化选择冰冻亚型红细胞融化洗涤后的保存条件及使用效期,保证其在血型血清学试验中的应用效果。方法从冰冻保存的亚型红细胞中,选择10份储存量较大、弱抗原与抗血清的反应强度为2﹢~3﹢的标本,融化洗涤后将压积红细胞各一分为二,分别以AB型血浆和MAP红细胞保存液按1.5倍的比例悬浮,加入终浓度为0.05mg/mL的硫酸庆大霉素混匀后,再各等分为1、2、3、4四组,第1组始终放置2~6℃冰箱保存,第2、3、4组每天在室温分别放置0.5、1和2h后,再放入2~6℃冰箱保存,于保存0、10、20、30、40、50、60d时,分别检测四组红细胞上清中的FHb含量及红细胞A、B抗原的反应强度。以红细胞每天置室温1h再恢复2~6℃储存的模式下,FHb浓度达1.0g/L,同时红细胞血型抗原减弱达1﹢的最小储存天数再减10d作为其使用效期。结果 (1)四组红细胞随着保存时间延长,上清FHb均逐渐升高;室温放置时间越长,上升幅度越大;以MAP作为保存介质,每天室温分别放置0、0.5、1、2h,FHb达到1.0g/L的时间分别为>60d、60d、50d、30d;以AB型血浆作为保存介质,则分别为50、40、30、10d。(2)四组红细胞随着保存时间延长,红细胞血型抗原的反应强度逐渐减弱;室温放置时间越长,抗原出现减弱的时间越早;以MAP作为保存介质,每天室温分别放置0、0.5、1、2h,血型抗原减弱达到1﹢的时间分别为>60、>60、60、40d;以AB型血浆作为保存介质,则分别为>60、60、50、20d。结论冰冻亚型红细胞融化洗涤后的保存质量与保存介质、温度、时间密切相关。以MAP红细胞保存液作为保存介质,置2~6℃冰箱储存,室温下使用时间每天控制在1h以内为其最佳保存条件;以MAP红细胞保存液作为保存介质的使用效期确定为40d;以血浆作为保存介质的使用效期则确定为20d。更多还原

【Abstract】 Objective To optimize the preservation conditions and validity period of frozen subgroup red blood cells after melting and washing to ensure its effects in blood group serology test. Methods 10 samples with large storage capacity and weak antigenicity and antiserum reaction intensity of 2+-3+ were selected from frozenly preserved subgroup red blood cells. After melting and washing, the packed red blood cells were divided into two shares which were mixed with 1.5 times of AB subgroup plasma and MAP red blood cell preservation fluid for suspension. Adding gentamicin sulfate with final concentration of 0.05mg/mL then equally diving each of the two shares into four groups. Group 1 was preserved in the fridge under temperature of 2-6℃, and Group 2, 3 and 4 were kept under room temperature for 0.5h, 1h and 2h each day before preservation in the fridge under temperature of 2-6℃. The supernatant FHb content and the reaction intensity of red blood cell antigen A and B were tested at 0d, 10 d, 20 d, 30 d, 40 d, 50 d and 60 d after preservation. With red blood cells kept under room temperature for 1h each day before preservation in the fridge under temperature of 2-6℃, the validity period was defined as the minimal storage days(for FHb concentration to reach 1.0g/L and the blood group antigen reaction intensity to decrease to 1+) minus 10 d. Results(1) The supernatant FHb content gradually increased with prolonged preservation of red blood cells, the longer preservation under room temperature, the greater increase. With MAP as the preservation medium, it took > 60 d, 60 d, 50 d, 30 d for FHb to reach 1.0g/L for red blood cells kept under room temperature for 0h, 0.5h, 1h and 2h respectively. With AB subgroup plasma as the preservation medium, it took 50 d, 40 d, 30 d and 10 d respectively.(2) Blood group antigen reaction intensity gradually decreased with prolonged preservation of red blood cells, the longer preservation under room temperature, the earlier appearance of decreased antigen reaction intensity. With MAP as the preservation medium, it took > 60 d, > 60 d, 60 d, 40 d for the blood group antigen reaction intensity to decrease to 1+ for red blood cells kept under room temperature for 0h, 0.5h, 1h and 2h respectively. With AB subgroup plasma as the preservation medium, it took>60d, 60 d, 50 d, s0 d respectively. Conclusion The preservation quality of frozen subgroup red blood cells after melting and washing is closely related to the preservation medium, temperature and time. The optimal preservation conditions are as follows: using MAP red blood cell preservation fluid as the preservation medium, preservation in fridge under temperature of 2-6 ℃, application under room temperature for no more than 1h each day. The validity period was defined as 40 d with MAP red blood cell preservation fluid as the preservation medium and 20 d with plasma as the preservation medium.更多还原