【摘要】 目的探索甲型副伤寒沙门菌(SPA)人群本底、恢复期病人及携带者脂多糖特异性抗体(LPS Ig G)血清抗体水平,为使用LPS Ig G抗体筛查方法鉴别甲型副伤寒携带者提供经验。方法在桂林等7个市、县筛选出440名有明确甲型副伤寒发病史的研究对象,采集其血液标本及粪便标本,使用ELISA方法检测血清LPS Ig G抗体滴度,对粪便标本进行SPA分离培养。把血清抗体结果与粪便SPA分离培养结果进行比较,了解不同特征对象的LPS Ig G抗体水平。结果共完成353名研究对象的随访,通过两轮粪便采集从7名对象中分离出SPA,其中6名为SPA携带者,1名为SPA慢性携带者。SPA阴性和阳性对象LPS Ig G平均抗体滴度分别为15.8 EU和103.5 EU,SPA阳性对象LPS Ig G平均抗体滴度高于阴性对象,差异有统计学意义(P=0.000)。以LPS Ig G抗体滴度≥35 EU作为阈值来筛查SPA阳性对象的灵敏度和特异度分别为86%和89%。结论 SPA携带者的LPS Ig G血清抗体水平远高于非携带者。通过LPS抗体检测能有效筛查出SPA携带者。更多还原

【Abstract】 Objective To determine the average titre of anti-LPS(Lipopolysaccharide) Ig G in native persons,historical SPA patients and paratyphoid A carriers and to determine the utility of screening with anti-LPS antibodies to identify paratyphoid carriers. Methods 440 target persons who have had an acute paratyphoid A fever episode confirmed by bacterial culture in the last 10 years were identified from 7 cities in Guangxi. Each subject was asked to provide blood and stool samples. Stool samples were performed by culture to isolate SPA and blood samples were performed by ELISA test to get anti-LPS Ig G titre. The results of stool culture and ELISA test were compared to determine anti-LPS Ig G titre between different groups.Results Among 353 target persons followed up 7 SPA strains were isolated from the stool samples which were collected in two rounds. Of them 6 target persons were identified as shedder and one as chronic carrier according to this study definition. The average Ig G Anti-LPS antibody titre of stool culture negative group and positive group was 15.8EU and103.5EU respectively. The average titre of anti-LPS Ig G of culture positive was significant higher than that culture negative. Anti-LPS antibody titers≥35EU had a 86%sensitivity and 89% specificity for detecting stool culture positive. Conclusion The anti-LPS Ig G of SPA carrier was far higher than non-SPA carrier. It was able to detect SPA carrier effectively by ELISA anti-LPS Ig G screening test.更多还原