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不同还原剂预处理精子对水牛ICSI介导转基因效果影响的初步研究
Effects of Sperm Pretreatment with Different Reducing Agents on the ICSI-Mediated Gene Transfer of Water Buffalo
【摘要】 本研究探讨了还原剂β-巯基乙醇(β-ME)、二硫苏糖醇(DTT)及谷胱甘肽(GSH)预处理水牛精子对水牛ICSI介导转基因(ICSI-mediated gene transfer,ICSI-Tr)效果的影响。结果显示,β-ME、DTT和GSH预处理水牛精子对提高ICSI介导转基因的效果不显著(p>0.05),但GSH能显著提高2原核(PN)的形成率(p<0.05)。
【Abstract】 Effects of sperm pretreatment with reducing agents(β-mercaptoethanol, β-ME; Dithiothreitol, DTT;Glutathione, GSH) on efficiency of ICSI-Tr in water buffalo were investigated. The results showed that, the sperm pretreatment of the three reducing agents could not improve the efficiency of ICSI-Tr in buffalo(p>0.05). But the pretreatment of GSH could significantly improve the 2PN formation rat(p<0.05).
【关键词】 水牛;
ICSI介导转基因;
还原剂;
精子;
【Key words】 Buffalo; ICSI-mediated gene transfer; Reducing agent; Sperm;
【Key words】 Buffalo; ICSI-mediated gene transfer; Reducing agent; Sperm;
【基金】 国家高技术研究发展计划(863)项目(2011AA100607);国家转基因重大专项(2011ZX08007-003)共同资助
- 【文献出处】 基因组学与应用生物学 ,Genomics and Applied Biology , 编辑部邮箱 ,2014年03期
- 【分类号】S823.83
- 【下载频次】60