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Superresolution imaging of DNA tetrahedral nanostructures in cells by STED method with continuous wave lasers

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【Author】 Jiancong Du;Suhui Deng;Shangguo Hou;Lingling Qiao;Jianfang Chen;Qing Huang;Chunhai Fan;Ya Cheng;Yun Zhao;College of Life Sciences, SiChuan University;Division of Physical Biology and Bioimaging Center, Shanghai Synchrotron Radiation Facility,Shanghai Institute of Applied Physics, Chinese Academy of Sciences;Shanghai Institute of Optics and Fine Mechanics,Chinese Academy of Sciences;

【机构】 College of Life Sciences, SiChuan University； Division of Physical Biology and Bioimaging Center, Shanghai Synchrotron Radiation Facility,Shanghai Institute of Applied Physics, Chinese Academy of Sciences； Shanghai Institute of Optics and Fine Mechanics,Chinese Academy of Sciences；

【摘要】 DNA tetrahedral nanostructures are considered to be new nanocarriers because they can be precisely controlled and hold excellent penetration ability to the cellular membrane.Although the DNA tetrahedral nanostructure is extensively studied in biology and medicine,its behavior in the cells with nanoscale resolution is not understood clearly.In this letter,we demonstrate superresolution fluorescence imaging of the distribution of DNA tetrahedral nanostructures in the cell with a simulated emission depletion(STED)microscope,which is built based on a conventional confocal microscope and can provide a resolution of 70nm.更多还原

【Abstract】 DNA tetrahedral nanostructures are considered to be new nanocarriers because they can be precisely controlled and hold excellent penetration ability to the cellular membrane. Although the DNA tetrahedral nanostructure is extensively studied in biology and medicine, its behavior in the cells with nanoscale resolution is not understood clearly. In this letter, we demonstrate superresolution fluorescence imaging of the distribution of DNA tetrahedral nanostructures in the cell with a simulated emission depletion(STED)microscope, which is built based on a conventional confocal microscope and can provide a resolution of 70nm.更多还原

【关键词】 tetrahedral； DNA； understood； depletion； letter； confocal； precisely； clearly； penetration； photonics；

【基金】 supported by the National Natural Science Foundation of China under Grand Nos.61008056,21227804,61078016,and 61378062)

【文献出处】 Chinese Optics Letters ,中国光学快报(英文版) , 编辑部邮箱 ,2014年04期

【分类号】Q751
【被引频次】4
【下载频次】96

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