【摘要】 目的研究硬棘软珊瑚醋酸乙酯部位的化学成分。方法采用正、反相硅胶柱色谱以及半制备高效液相色谱进行分离,并根据理化性质和波谱数据鉴定化合物的结构。结果分离得到11个化合物,其中3个环丁烯内酯类化合物,分别鉴定为(S)-13-(2-羟基-3,4-二甲基-5-氧代-2,5-二氢呋喃-2-基)十三烷酸(1)、(S)-11-(2-羟基-3,4-二甲基-5-氧代-2,5-二氢呋喃-2-基)十一烷酸(2)、羟基二氢博伏内酯(3);1个嘌呤类化合物,鉴定为1,3-二甲基黄嘌呤(4);7个甾体类化合物,分别鉴定为孕甾-20-烯-3-酮(5)、孕甾-1,4,20-三烯-3-酮(6)、3β-羟基-孕甾-20-烯(7)、3β-羟基-孕甾-5,20-二烯(8)、20-羟基-孕甾-1,4-二烯-3-酮(9)、20-羟基-孕甾-1-烯-3-酮(10)、17β-羟基-雄甾-1-烯-3-酮(11)。结论环丁烯内酯类化合物是首次从该属软珊瑚中分离得到,其中化合物1和2为新化合物,而硬棘软珊瑚醋酸乙酯部位的化学成分主要为甾体类化合物。更多还原

【Abstract】 Objective To investigate the chemical constituents in the ethyl acetate fraction from Scleronephthya sp.. Methods The compounds were isolated by column chromatography(silica gel, including reversed phase) and semi-prepative HPLC, and their structures were identified on the basis of physicochemical properties and spectroscopic data. Results Eleven compounds were separated, three of which were butenolides, identified as(S)-13-(2-hydroxy-3,4-dimethyl-5-oxo-2,5-dihydrofuran-2-yl) tridecanoic acid(1),(S)-11-(2-hydroxy-3,4-dimethyl-5-oxo-2,5-dihydrofuran-2-yl) undecanoic acid(2), and hydroxydihydrobovolide(3), one of which was purine, identified as 1,3-dimethylxanthine(4), seven of which were pregnane steroids, identified as pregna-20-en-3-one(5), pregna-1,4,20-trien-3-one(6), 3β-hydroxy-pregna-20-ene(7), 3β-hydroxy-pregna-5,20-diene(8), 20-hydroxy-pregna-1,4-dien- 3-one(9), 20-hydroxy-pregna-1-en-3-one(10), and 17β- hydroxy-androst-1-en-3-one(11). Conclusion Pregnane steroids are the main chemical constituents in ethyl acetate fraction of Scleronephthya sp., and compounds 1 and 2 are new compounds, while butenolides are obtained from Scleronephthya sp. for the first time.更多还原