【摘要】 目的采用表面等离子体共振(surface plasmon resonance,SPR)技术研究壳聚糖纳米粒与细胞膜的相互作用。方法利用SPR细胞传感器动态监测壳聚糖及胸腺五肽作用于C6细胞诱发的折射率变化。结果壳聚糖纳米粒或壳聚糖分子与C6细胞间具有很强的相互作用,折射率变化较大,在作用30 min内,SPR信号升高并且具有浓度依赖性。但胸腺五肽分子与C6细胞间无相互作用。结论 SPR技术能够实时在线地表征纳米粒与生物膜的相互作用。更多还原

【Abstract】 Objective Using surface plasmon resonance(SPR)technology to monitor the interactions between chitosan nanoparticles(CS-NP) and biomembrane. Methods SPR signals induced by CS-NP,chitosan molecules and thymopentin(TP5)molecules in living C6 cells were detected and analyzed by the SPR-based cytosensor. Results The signal began to increase within30 min after the addition of CS-NP or chitosan molecules. These responses were strongly dependent on the concentrations of CS.However,the signal did not change after the addition of TP5 molecules. Conclusion SPR technology can clarify the interaction between nanoparticles and biomembrane in real time.更多还原