【摘要】 为掌握上海市沙门氏菌在农贸市场和超市禽肉制品和活禽中的污染状况、流行血清型和耐药情况,本试验从320份2012年采集的禽肉样本(240份)和活禽泄殖腔棉试子(80份)中共分离鉴定出沙门氏菌70株,阳性率为21.9%。血清型鉴定结果表明,共鉴定出11种不同的血清型,其中肠炎沙门氏菌占47.1%、印第安纳沙门氏菌占17.1%、鼠伤寒沙门氏菌和德尔卑沙门氏菌均占8.6%。通过纸片法对这70株沙门氏菌进行了16种常用抗菌药物的药敏试验,结果表明,沙门氏菌对奈啶酸耐药率最高,达75.7%,对磺胺异唑、链霉素和氨苄西林耐药率较高,分别为60.0%、60.0%和51.4%。除亚胺培南外,其他药物均有不同程度的耐药菌株。有40株(57.1%)沙门氏菌呈多重耐药表型,耐药性最强的菌株可以对14种抗菌药产生耐药性。本研究对沙门氏菌病的防控及指导临床合理用药具有重要的现实意义。更多还原

【Abstract】 The purpose of this study was to understand the pollution condition,popular serotype and drug resistance of Salmonellain farmer’s market and supermarket,in Shanghai.A total of 240 chicken samples and 80chickens’ cloaca swabs were obtained from market in 2012.Among them,70(21.9%)were positive for Salmonella.Of the 11 serotypes in 70 Salmonella isolates,S.enteritidis(47.1%),S.indiana(17.1%),S.typhimurium(8.6%)and S.derby(8.6%)were prevalent.Drug susceptibility test was conducted among the Salmonellaisolates using the Kirby-Bauer disk diffusion method.The highest level of resistance was found for nalidixie acid(75.7%),followed by sulfisoxazole(60.0%),streptomycin(60.0%)and ampicillin(51.4%).No resistance to imipenem was observed in any isolate examined.The isolated chicken Salmonellastrains had severe resistance to most drugs.40 strains of Salmonella were millti-drug resistant,the most resistart strain was resistant to 14 drugs.This study had important practical significance for prevention and control and rational drug use of salmonellosis.更多还原