【摘要】 将纯化后的猪瘟病毒免疫4只SPF级BALB/c小鼠,无菌取脾细胞与SP2/0骨髓瘤细胞融合,经半固体培养基克隆化和间接ELISA筛选,最终获得了4株稳定分泌抗猪瘟病毒单克隆抗体的杂交瘤细胞株。ELISA结果显示4株单克隆抗体效价均较高,并与其他病毒及PK-15细胞培养物无交叉反应。Western blotting结果证实3株单克隆抗体可特异性识别猪瘟病毒。间接免疫荧光试验可在细胞膜内观察到特异性绿色荧光,表明3株单克隆抗体与猪瘟病毒具有良好的反应性。该研究为猪瘟病毒新型诊断试剂开发奠定了基础。更多还原

【Abstract】 4 SPF BALB/c mice were immunized with purified classical swine fever virus(CSFV)as the antigen and mouse splenic cells were fused with SP2/0 cells.Hybridoma cells were screened by ELISA and semi-solid agar cloning,4 hybridoma cells secreting anti-CSFV monoclonal antibodies were obtained.ELISA results showed that all of these monoclonal antibodies with high antibody titer,which could specifically react to CSFV but not cross react to other pig viruses and PK-15 cell cultures.Western blotting indicated that 3 strains of monoclonal antibodies reacted with CSFV specially.Specific green fluorescence was observed in the cell membrane by IFA.This study layed a foundation for the development of new diagnostic reagents for CSFV.更多还原