【摘要】 根据水貂阿留申病毒（Aleutian mink disease virus,AMDV）基因序列（GenBank登录号:NC₀01662.1）设计1对特异性引物,通过对荧光定量PCR反应条件的优化,建立了检测AMDV的SYBR GreenⅠ荧光定量PCR方法,该方法的灵敏度达10拷贝/μL,≥102拷贝/μL具有良好的特异性和重复性。同时利用该方法对8份疑似AMDV血清进行检测,结果6份阳性,阳性率为75%。本研究为水貂阿留申病的鉴别诊断及净群根除奠定了技术基础。更多还原

【Abstract】 One pair of specific primers was designed and synthesized according to the sequence of Aleutian mink disease virus(AMDV)subbitted in the GenBank database,and then reaction requirements were optimized to develop a SYBR GreenⅠ fluorescence quantitative PCR assay.The results showed that the fluorescence quantitative PCR assay could detect10copies/μL of plasmid DNA.Its specificity and reproducibility were very good when 102 copies/μL.Meanwhile,8field samples were detected with the methods and the positive rate was 75%.This study would lay the foundation for differentiation diagnosis and purification of the mink population.更多还原