In this study,Altay sheep was used for investigating the association between sheep tenderness and single nucleotide polymorphism markers on the calpastatin(CAST)gene.Its genetic polymorphism was detected using SSCP and DNA sequencing technology in exon 1,exon 2and exon 21.However,only three genotypes AA,AB and BB were detected in exon 21,the sequencing result showed that the mutation of A into G,association analysis indicated that the mutation in exon 21affected the meat tenderness markedly of Altay sheep(P...
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