【摘要】 本试验旨在研究不同剂量苦马豆素(SW)胃内灌服小白鼠,检测其对肝脏α-甘露糖苷酶(AMA)基因在不同时间转录表达的影响。采用动物组织总RNA试剂盒提取肝脏组织总RNA,酶标仪法测定RNA纯度,凝胶电泳检测RNA完整性,实时荧光定量PCR技术检测AMA基因的转录表达水平。结果显示,试验组及对照组AMA基因均有表达,但各试验组中转录表达水平不同,与对照组相比,低剂量SW能促进肝脏AMA基因的表达,且随着试验的进行促进效果更加明显,高剂量和中等剂量SW初期也能提高AMA基因的相对表达,长时间却能严重抑制AMA基因的表达,随着试验的进行抑制效果愈明显。结果表明,不同剂量SW能在不同时间、不同程度地影响小白鼠肝脏AMA基因的转录表达。更多还原

【Abstract】 This study was aimed to investigateα-mannosidase(AMA)gene transcription at different time by intragastric drenching different doses of swainsonine(SW)to mice.Animal total RNA extraction kit,microplate method,gel electrophoresis and Real-time quantitative PCR were used in this experiment to extract liver total RNA and detect RNA purity,RNA integrity and AMAgene transcription.The results showed that AMAgene of the experimental groups and control group were expressed and different experimental groups had different transcription levels.Compared with control group,low doses of SW could promote AMAgene expression and the promotion gradually increased as the trial went on.However,high and middle doses of SW could promote AMAgene expression in initial stage and inhibit it seriously in long time and the inhibition gradually increased as the trial went on.The results showed that different doses of SW had effect on mouse liver AMAgene transcription and expression at different time and different levels.更多还原