【摘要】 为比较家兔与BALB/c小鼠对猪瘟病毒E2蛋白靶向化树突状细胞疫苗的敏感性,本研究首先根据小鼠与家兔体重比,分别免疫糖基化E2蛋白与E2蛋白(家兔2mg/只,小鼠20μg/只),在不同时间采血制备血清,通过ELISA方法测定血清中IgG水平。结果显示,接种后BALB/c小鼠出现了轻度的免疫应答,而家兔产生了强而持久的免疫保护。本研究对BALB/c小鼠的接种量进行了进一步摸索,结果表明,50与100μg/只免疫效果明显优于20μg/只;其中50μg/只E2蛋白与糖基化E2蛋白免疫后,血清IgG水平较高且稳定;而100μg/只糖基化E2蛋白免疫后,免疫初期,糖基化E2蛋白组产生了免疫抑制,至21d抗体水平逐渐升高且达极高水平;但100μg/只E2蛋白免疫后,整个免疫期内抗体水平均较低,只有轻微波动。以上结果表明,家兔对糖基化E2蛋白与E2蛋白的敏感性均显著高于BALB/c小鼠,且糖基化E2蛋白免疫效果明显好于E2蛋白,BALB/c小鼠的糖基化E2蛋白最佳免疫剂量为50μg/只。更多还原

【Abstract】 In order to compare the sensitivity to classical swine fever virus E2protein targeting dendritic cell vaccine between rabbits and BALB/c mice,we firstly inoculated rabbits and BALB/c mice with glycosylated E2protein and E2protein(2mg in rabbits and 20μg in mice)according to the body weight percentage of rabbits and BALB/c mice.The IgG levels in the serum were determined by ELISA method in different time.The results showed that the BALB/c mice generated mild immune response only,and the rabbits had a strong and lasting immune protection.Secondly,the research on inoculation ammount in BALB/c mice were explored further.The results indicated that the immune effects were obviously better after inoculation with 100or 50μg than that of 20μg inoculation amount.The IgG level in serum was high and stable after the BALB/c mice inoculated with 50μg glycosylated E2protein.However,the mice emerged immune suppression in initial stage after inoculation with 100μg glycosylated E2protein,and the IgG level increased to high level on 21d.Surprisingly,the IgG level was low and only slightly fluctuated after the mice inoculated with 100μg E2protein.The above results proved that the sensitivity of rabbits was significantly higher than that of BALB/c mice to the glycosyated E2protein and E2protein.Furthermore,the immune effect of glycosylated E2protein was better than that of E2protein,and the best inoculation dose was 50μg in BALB/c mice.更多还原