【摘要】 目的探讨丙型肝炎病毒核酸和抗体检测方法在人群筛查中的应用。方法采用胶体金快速试验法和酶联免疫吸附试验(ELISA)检测丙型肝炎病毒(HCV)抗体,实时荧光定量PCR(RT-PCR)检测HCV-RNA病毒载量。结果 (1)539份样本中,其中266例抗体阴性,263例抗体阳性。(2)在67例HCV-RNA病毒载量小于103 IU/mL组中,ELISA法检测HCV抗体阳性有60例,胶体金快速试验法检测抗体阳性有30例。208例HCV-RNA病毒载量大于或等于103 IU/mL组中,ELISA法检测的抗体阳性有199例,胶体金快速法检测阳性的有181例,另外有6例两种抗体检测均为阴性患者RNA病毒载量大于或等于103 IU/mL。(3)对208例HCV-RNA病毒载量大于或等于103 IU/mL样本分成4个组。GGT、ALT及AST在4组间差异均有统计学意义(P<0.05),而ALB及S/CO值在4组间差异无统计学意义(P>0.05)。结论在人群筛查中为了减少漏诊率,尽早诊断丙型肝炎,要联合运用以上各实验室检测方法,综合分析。更多还原

【Abstract】 Objective To investigate the application of hepatitis C virus RNA and antibody detection method in population screening.Methods The colloidal gold rapid test method and the enzyme-linked immunosorbent assay(ELISA)were adopted to detect hepatitis C virus(HCV)antibodies,and the real-time quantitative PCR(RT-PCR)was adopted to detect HCV-RNA viral load.Results(1)Among 539 samples,266cases were antibody negative and 263 cases were antibody positive.(2)Among 67 cases in the HCV-RNA viral load<103 IU/mL group,60 cases were HCV antibody positive by ELISA and 30 cases were HCV antibody positive by colloidal gold rapid test.Among 208 cases in the HCV-RNA viral load≥103 IU/mL,199 cases were antibody positive by ELISA,but only 181 cases were antibody positive by the colloidal gold rapid method.Other 6cases of were 2kinds of antibody negative had the HCV-RNA viral load≥103 IU/mL.(3)208cases of HCV-RNA viral load≥103 IU/mL sample were divided into four groups.GGT,ALT and AST were statistically significantly different P<0.05),while ALB and S/CO values hadno statistical difference(P>0.05).Conclusion In order to reduce the missed diagnosis rate and diagnose hepatitis C as early as possible,the above laboratory detection methods should be jointly applied and the comprehensive analysis should be conducted in population screening.更多还原