【摘要】 目的评价多台血液分析仪检测结果的一致性。方法采用NCCLS文件EP9-A2指南、Passing-Bablok回归分析法和t检验,使用115例患者标本对5台血液分析仪(XE-2100,XT-1800i,XS-1000i,BC-5300,ABX Pentra80)的血红蛋白(HGB)、红细胞压积(HCT)、红细胞计数(RBC)、白细胞计数(WBC)、血小板计数(PLT)进行了比较,其中Sysmex XE-2100血液分析仪作为参比仪器。结果在所有比对项目HGB、HCT、RBC、WBC、PLT中,这几台血液分析仪显示出了非常好的相关性(r>0.97),但有3台血液分析仪的个别项目产生了比例系统误差和/或恒定系统误差,包括XT-1800i血液分析仪的HCT项目,XS-1000i血液分析仪的HCT、RBC项目,ABX80血液分析仪的RBC项目。结论通过Passing-Bablok回归分析法联合t检验能识别出两台仪器比对结果的系统误差,不可以使用简单的相关系数评价血液分析仪比对结果的一致性。更多还原

【Abstract】 Objective To evaluate the consistency of the test results detected by multiple automated hematology analyzers.Methods The EP9-A2 guideline of NCCLS,Passing-Bablok regression analysis and t test were adopted to compare the detection results of hemoglobin(HGB),hematocrit(HCT),red blood cell count(RBC),white blood cell count(WBC)and platelet(PLT)in 115 samples from patients by 5 automated haematology analyzers of the Sysmex XE-2100,the Sysmex XT-1800i,the Sysmex XS-1000i,the Mindray BC-5300 and ABX pentra80.The Sysmex XE-2100 was used as the reference instrument.Results In the detection of all the compared items including HGB,HCT,RBC,WBC and PLT,these automated hematology analyzers exhibited very good correlation(r>0.97),whereas the individual parameters in 3 automated hematology analyzers appeared the proportional systematic differences and/or constant systematic differences,including HCT in the sysmex XT-1800i,HCT and RBC in the sysmex XS-1000i and RBC in the ABX pentra 80.Conclusion The Passing-Bablok regression analysis combined with t test may identify the systematic differences of the comparative results of 2 automated haematology analyzers,the consistency of the comparative results of the automated hematology analyzers can not be evaluated by the simple correlation coefficient.更多还原