【摘要】 目的探讨间接免疫荧光法检测抗核抗体(ANA)结果与蛋白质印迹法检测ANA结果之间的相关性,以及两种方法联合检测在自身免疫性疾病中的诊断价值。方法收集2011年4月至2013年4月同时用间接免疫荧光法与蛋白质印迹法检测ANA的患者资料,并对检测结果进行对比分析。结果间接免疫荧光法检出ANA阳性率明显低于蛋白质印迹法(χ2=158.69,P<0.01)。间接免疫荧光法检测ANA阳性标本中,有20例蛋白质印迹法检测为阴性;蛋白质印迹法检测ANA为阳性的775例标本中,有295例(38.06%)间接免疫荧光法检测为阴性。间接免疫荧光法检测ANA的荧光模型与特异性ANA之间没有绝对的规律。结论蛋白质印迹法检测ANA的敏感性较高,间接免疫荧光存在假阴性的可能,选择一个敏感性较高的检测组合十分必要。更多还原

【Abstract】 Objective To investigate the correlation between indirect immunofluorescence(IFT)method and Western blot assay for the diagnosis of antinuclear antibody(ANA),based on which to evaluate the diagnostic value of IFT combined with Western blot for the autoimmune disease.Methods Medical materials from patients received IFT combined with Western blot for the diagnosis of autoimmune disease from April 2011 to April 2013 were included in this study.On this basis,a comparative study was performed to evaluate the diagnosis results.Results The positive rates obtained using IFT method were significantly lower than that of Western blot assay(χ 2=158.69,P<0.01).Among the samples with positive results using IFT method,20 samples were considered as negative using Western blot assay.On the contrary,among the 775 samples with positive results using Western blot assay,295 samples(38.06%)were considered to be negative using IFT method.No absolute association was established between the fluorescent pattern and specificity of the IFT method in the diagnosis of ANA.Conclusion The sensitivity of Western blot assay for the diagnosis of ANA was comparatively higher than that of IFT method.A possibility of false negative was noted in the IFT method.It is necessary to select a combined method with higher sensitivity for the diagnosis of ANA.更多还原