【摘要】 目的了解尿标本中临床分离菌的分布及耐药性。方法采用仪器法和纸片扩散法测定抗菌药物敏感性,参照美国临床实验室标准化研究所(CLSI)M100-S22文件标准判读结果,用WHONET5.6软件统计分析。结果 2012年尿标本非重复临床分离株2 019株。其中革兰阴性菌1 589株(占78.7%),革兰阳性菌430株(占21.3%);分离的菌株中以大肠埃希菌为主(占43.2%),其次为肠球菌属(占14.9%);约59%的菌株分离自女性患者,41%的菌株分离自男性患者;大肠埃希菌和肺炎克雷伯菌超广谱β-内酰胺酶(ESBLs)的检出率分别为58.9%和52.6%。肠杆菌科细菌对亚胺培南和厄它培南出现耐药株,阴沟肠杆菌对亚胺培南和厄他培南的耐药率分别达14.1%和40%。检出三株万古霉素耐药的屎肠球菌(占2.3%)。结论尿标本的主要分离菌为大肠埃希菌,其次肠球菌属细菌和肺炎克雷伯菌。阴沟肠杆菌对碳青霉烯类抗菌药物耐药率呈明显升高趋势。更多还原

【Abstract】 Objective To investigate the antibiotic resistance of clinical isolates from urine specimens to antimicrobial agents commonly used during 2012.Methods Automatic clinical microbiological system and disk diffusion method was performed to determine the antimicrobial susceptibility.All datum were analyzed by WHONET5.6software according to the breakpoints of Clinical Laboratory Standard Institution(CLSI)M100-S22.Results A total of 2 019clinical isolates were collected from urine specimens in 2012,including gram-negative bacilli 78.7%(1589/2 019)and gram-positive cocci 21.3%(430/2 019).Escherichia coli was the most prevalent species(43.2%),followed by Enterococcus(14.9%).About 59% of the strains were isolated from female patients, 41%form male patients,Escherichia coli and Klebsiella pneumoniae extended spectrum beta lactamases(ESBLs)detection rate was 58.9%and 52.6%,respectively.Some enterobacteriaceae strains were resistant to imipenem or ertapenem.The resistance rates of Enterobacter cloacae to imipenem and ertapenem were 14.1%and 40.0%.Three strains of Enterococcus faecium was found resistant to vancomycin.Conclusion Escherichia coli is the leading clinical isolate in urine,followed by Enterococcus and Klebsiella pneumoniae.Antimicrobial drug resistance rate is significantly increased trend of Enterobacter cloacae to carbapenems.更多还原