【摘要】 目的评价Cobio XS和Sysmex UF-1000i2种尿沉渣分析仪检测红白细胞的可靠性。方法选取红细胞和白细胞在高、中、低3个不同浓度水平的尿液标本在Cobio XS和Sysmex UF-1000i尿沉渣分析仪检测,测定2台仪器的重复性。将新鲜血标本稀释成不同的浓度,测定2台仪器的稀释线性。收集住院患者尿液标本176份,以FAST-READ 102沉渣计数板结果为标准,统计2种仪器的假阳性率,假阴性率。结果 Cobio XS尿沉渣分析仪经人工校正后高、中、低值3水平的白细胞重复性变异系数为5.53%、6.29%、4.21%,红细胞变异系数为3.47%、4.98%、3.32%。Sysmex UF-1000i尿沉渣分析仪白细胞的重复性变异系数为2.74%、2.31%、2.90%,红细胞变异系数为1.75%、1.26%、1.39%。Sysmex UF-1000i尿沉渣分析仪红细胞在7～10 210个/μL线性范围内的相关系数为0.999 9,Cobio XS尿沉渣分析仪红细胞在4～5 300个/μL线性范围内的相关系数为0.999 3。Sysmex UF-1000i尿沉渣分析仪红细胞白细胞的假阳性率分别为43.8%和20.5%,Cobio XS尿沉渣分析仪分别为3.4%和11.4%。Sysmex UF-1000i尿沉渣分析仪红细胞白细胞的假阴性率分别为0.6%和0.6%,Cobio XS尿沉渣分析仪分别为1.7%和1.7%。结论 Cobio XS和Sysmex UF-1000i尿沉渣分析仪均有良好的稀释线性。Sysmex UF-1000i尿沉渣分析仪的重复性较Cobio XS尿沉渣分析仪好,Sysmex UF-1000i尿沉渣分析仪红细胞白细胞的假阳性率明显高于Cobio XS尿沉渣分析仪,假阴性率稍低于Cobio XS尿沉渣分析仪。更多还原

【Abstract】 Objective To evaluate the reliability of the red/white blood cell test results on Cobio XS and Sysmex UF-1000iurinary sediment analyzer.Methods The precision and linearity of Cobio XS and Sysmex UF-1000iurinary sediment analyzer were measured with urine samples.The false positive rate and false negative rate of the two urinary sediment analyzer were measured.Results After the manual adjustment,the within run coefficients of variation(CV%)of Cobio XS urinary sediment analyzer on white blood cell in high,middle and low value samples were 5.53%,6.29%and 4.21%respectively.They were 3.47%,4.98%,3.32% for red blood cell respectively.The within run coefficients of variation(CV%)of Sysmex UF-1000isediment analyzer on white blood cell in high,middle and low value samples were 2.74%,2.31%and 2.90%respectively.They were 1.75%,1.26%,1.39% for red blood cell respectively.The correlation coemcient Sysmex UF-1000iurinary sediment analyzer of RBC was 0.9999with 7-10 210/μL.The correlation coemcient Sysmex UF-1000iurinary sediment analyzer of RBC was 0.9993with 4-5 300/μL.Compared to the result of FAST-READ 102urinary sediment quantitative counting plate,the false positive rate of red/white blood cell of Sysmex UF-1000iurinary sediment analyzer were 43.8%and 20.5%,which were 3.4%and 11.4% with Cobio XS,and the false negative rate of red/white blood cell of Sysmex UF-1000iUrinary Sediment Analyzer is 0.6% and 0.6%,which were 1.7% and 1.7% with Cobio XS,respectively.Conclusion Cobio XS and Sysmex UF-1000iurinary sediment analyzer are all good in linearity performance.The precision of Sysmex UF-1000iurinary sediment analyzer is better than that of Cobio XS.The false positive rate of red/white blood cell of Sysmex UF-1000iurinary sediment analyzer is significantly higher than that of Cobio XS.The false negative rate of red/white blood cell of Sysmex UF-1000iurinary sediment analyzer is slightly lower than that of Cobio XS.更多还原