【摘要】 目的比较抗核抗体谱-12S检测(antinuclear antibody profile,ANA-12S)试剂盒和抗核抗体谱线性免疫分析法检测(antinuclear antibody spectrum linear immunoassay,IMTEC-ANA-LIA)试剂盒对血清抗核抗体谱(antinuclear antibody spectrum,ANAs)检测结果的一致性,从而验证ANA-12S检测ANAs的有效性。方法收集于该院就诊的间接免疫荧光法(indirect immunofluorescence,IIF)检测抗核抗体(ANA)阳性患者标本144例,其中男21例,女123例;ANA阴性患者标本10例,其中男2例,女8例。用IMTEC-ANA-LIA和ANA-12S分别进行ANAs检测。比较和分析两种方法所得的检测结果。结果 ANA阳性标本中两者检测阳性率均为100%,其中ANA-12S法检测阳性项目占28.8%(497/1 728),IMTEC-ANA-LIA法检测阳性项目占27.7%(479/1 728),差异无统计学意义(χ2=0.46,P>0.05),ANA-12S法与IMTEC-ANA-LIA法检测结果总符合率为97.1%,Kappa值为0.929;ANA阴性标本中两者检测的阴性率均为70%(7/10),其中3例系统性红斑狼疮(SLE)疑似患者抗SmD1阳性,阴性项目占97.5%(117/120),总符合率100%,Kappa值为1。结论 ANA-12S与IMTEC-ANA-LIA的检测结果具有较高符合率和准确性;ANA-12S试剂盒可以作为快速、简便、经济的检测方法应用于自身抗体的检测。更多还原

【Abstract】 Objective To compare the consistency of serum antinuclear antibody spectrum detection by antinuclear antibody profile(ANA-12S)and antinuclear antibody spectrum linear immunoassay(IMTEC-ANA-LIA),which verifies the effectiveness of ANA-12Sassay for detecting antinuclear antibody spectrum.Methods 144antinuclear antibody positive specimens detected by indirect immunofluorescence(IIF)were collected from our hospital including 21males and 123females,and 10antinuclear antibody negative specimens were also collected including 2males and 8females.The antinuclear antibody spectrum results were then compared and analysed between the IMTEC-ANA-LIA and ANA-12Sassays.Results The positive rate of ANAs by both assays in antinuclear antibody positive specimens were 100%.Positive projects accounted for 28.8%(497/1728)by ANA-12Sassay,and accounted for 27.7%(479/1728)by IMTEC-ANA-LIA assay.There were no statistically significant differences(χ2=0.46,P> 0.05).The total coincident rate of the positive results by ANA-12Sassay compared to IMTEC-ANA-LIA was 97.1% and the Kappavalue was 0.929.The negative rate of ANAs by both assays in antinuclear antibody negative specimens was 70%(7/10). Three specimens from SLE suspects were detected SmD1antibodies positive and the negative projects accounted for 97.5%(117/ 120).The total coincident rate of the negative results was 100%and the Kappavalue was 1.Conclusion There is a high coincident rate and accuracy between the IMTEC-ANA-LIA and ANA-12Sassays.It is a simple,fast and economical method for detecting antinuclear antibody spectrum by ANA-12Sassay.更多还原