【摘要】 [目的]通过建立与番茄黄化曲叶病毒病抗病基因Ty-3紧密连锁的SCAR标记,以提高抗病材料选育效率与准确性。[方法]设计特异引物对相关材料进行PCR扩增,获得与抗病基因Ty-3连锁的SCAR分子标记。[结果]利用该标记检测抗病纯合材料(Ty-3/Ty-3)、感病材料(ty-3/ty-3)分别产生600 bp、320 bp片段,抗病杂合材料(Ty-3/ty-3)产生600 bp与320 bp 2条片段;利用该标记对7份田间表现抗病的番茄F1代杂交种进行检测,有4份含有抗病基因Ty-3,3份未检出;选择经济性状优良、含有抗病基因Ty-3的F1代杂交种自交得到F2群体28个单株,利用该标记对单株进行检测,得到抗病纯合单株7个,抗病杂合单株13个,感病单株8个。[结论]该标记是与抗病基因Ty-3紧密连锁的共显性标记,可用于番茄抗病基因Ty-3的鉴定与抗病材料的筛选。更多还原

【Abstract】 [Objective] The aim was to obtain SCAR marker tightly linked for resistance gene Ty-3 in tomato,which would increase the efficiency and accuracy of resistance materials. [Method] The specific primer was designed for PCR amplified. The SCAR molecular marker which was linked to Ty-3 was detected. [Result]With the SCAR markerm, resistant and susceptible homozygous materials produced about 600 bp and 320 bp PCR fragments respectively and resistant heterozygous produced 600/320 bp fragments. Ty-3 resistance gene of 7 F1 were detected with this marker, The results showed that 4 hybrids contained Ty-3, but 3 hybrids not.Though all of them showed resistance. The SCAR marker was also used to detect Ty-3 resistance gene of F2 individuals. The results showed that 7 individuals contained Ty-3/Ty-3, 13 individuals contained Ty-3/ty-3.[Conclusion] The SCAR marker which could distinguish resistant,susceptible and co-dominant marker tightly linked to Ty-3 gene and could be used for MAS.更多还原