【摘要】 运用激光共聚焦显微成像技术,通过应用腺苷受体的特异性阻断剂和激动剂,研究腺苷受体对腺苷诱导的大鼠背根神经节(DRG)神经元中Ca2+的动态变化的影响,并且进一步研究了腺苷诱导Ca2+动态变化的剂量相关性。结果显示,腺苷A1和A3受体参与腺苷诱导的DRG神经元Ca2+释放,腺苷能引起DRG神经元内Ca2+浓度升高且呈剂量相关性。研究结果表明,共焦显微成像能有效检测单细胞内离子的动态信号,有助于了解腺苷及其受体参与疼痛过程中细胞离子信号传递的机制。更多还原

【Abstract】 Adenosine receptors have been further subdivided into four subtypes,A1,A2A,A2B and A3,all of which couple to G proteins and have been cloned successfully.In order to investigate which adenosine receptors are involved in adenosine-induced cellular Ca2+dynamics in dorsal root ganglion(DRG)neurons and whether the effects of adenosine on intracellular Ca2+dynamics are dose-response,in this study we use selective agonists and antagonists of adenosine receptors by laser scanning confocal microscopy and Ca2+fluorescent dye Fluo 4/AM.Our results show that using adenosine A1and A3receptors selective antagonists could block adenosine-induced Ca2+dynamics,which is not found with adenosine A2A and A2B receptors selective antagonists.It shows that adenosine A1receptor(A1R)and adenosine A3receptor(A3R)might be related to adenosine-evoked Ca2+release in DRG neurons.In addition,we find that the adenosine stimulation of intracellular Ca2+concentration in DRG neurons is dose-response.This study suggests that the cellular ion signaling could be detected efficiently by laser scanning confocal microscopy.The results are helpful to reveal the role of adenosine and adenosine receptor in ion signal transmission during pain.更多还原