【摘要】 目的对2006—2012年深圳市南山区从食物中毒病例分离的副溶血性弧菌O3∶K6血清型进行病原学特征分析。方法收集深圳市南山区2006—2012年食物中毒腹泻病例分离的副溶血性弧菌O3∶K6血清型,采用多重PCR方法检测其毒力基因tdh和trh,采用脉冲场凝胶电泳(PFGE)进行分子分型,并采用K-B法进行药敏试验。结果共收集到2006—2012年食物中毒腹泻病例分离的44株副溶血性弧菌O3∶K6,全部携带tdh基因,不携带trh基因。PFGE显示,44株菌可分为2个克隆群,克隆群1(C1)有19株菌,每株菌有17~20个条带,克隆群2(C2)有25株菌,每株菌有17~18个条带,C1和C2在200、190和100 Kb 3个条带处有差异,其他条带基本相同。药敏结果显示,44株副溶血性弧菌O3∶K6对头孢他啶、头孢吡肟、庆大霉素、左氧氟沙星、四环素、氯霉素和复方新诺明100.00%敏感,对氨苄青霉素、卡那霉素、链霉素的耐药率分别为72.73%(32/44)、18.18%(8/44)、18.18%(8/44),中度敏感率分别为9.09%(4/44)、65.91%(29/44)、65.91%(29/44)。结论该地区从食物中毒腹泻病例分离的副溶血性弧菌O3∶K6主要的克隆群为C1和C2,携带tdh基因,不携带trh基因。更多还原

【Abstract】 Objective To characterize O3∶ K6 serotype of V. parahaemolyticus isolated from cases of food poisoning during 2006-2012 in Nanshan District,Shenzhen. Methods Strains of V. parahaemolyticus serotype O3∶ K6 were collected. Multi-PCR,pulsed field gel electrophoresis( PFGE),and KirbyBauer were performed. Results A total of 44 strains of V. parahaemolyticus( O3∶ K6) obtained from diarrhea patients with food poisoning were collected. All strains were tdh gene positive and trh gene negative.PFGE indicated that the 44 strains could be divided into 2 clone complexes. Clone 1( C1) had 19 strains,with 17-20 bands for each strain,while clone 2( C2) had 25 strains,with 17-18 bands for each one.Differences between C1 and C2 were found in position of 200,190,and 100 Kb,while the other bands were similar. All the 44 strains( 100. 00%) were sensitive to cefrazidime,cefepime,gentamicin,levoflocacin,tetracycline,chloramphenicol,and sulphamethoxazole,72. 73%( 32 /44),18. 18%( 8 /44),and18. 18%( 8 /44) of strains were resistant to ampicillin,kanamycin,and streptomycin,and 9. 09%( 4/44),65.91%( 29/44),and 65.91%( 29/44) of strains were moderately sensitive to the three antibiotics,respectively. Conclusion The main PFGE clone complexes of strains of V. parahaemolyticus serotype O3∶ K6 isolated in the local area were C1 and C2,with tdh gene positive and trh gene negative.更多还原