【摘要】 目的观察肺炎支原体感染对BALB/c小鼠气道IFN-γ和IL-4、IL-5、IL-10以及气道阻力的影响。方法建立肺炎支原体经呼吸道感染的BALB/c小鼠模型,应用RT-PCR和HE染色的方法进行模型的鉴定,用ELLSA的方法检测BALF中细胞因子的含量,AniRes2005动物肺功能仪测各组小鼠肺的吸气阻力、呼气阻力、顺应性。结果 MP感染BALB/c小鼠后IFN-γ和IL-4、IL-5、IL-10细胞因子较正常对照组明显升高(P<0.05);吸气阻力、呼气阻力较正常对照组增加(P<0.05),顺应性降低较正常对照组增加(P<0.05)。结论 MP肺炎感染BALB/c小鼠后气道阻力增加、顺应性降低,而且气道中的炎性因子持续存在。更多还原

【Abstract】 Objective To study the the effects of mycoplasma pneumonia(MP) infection on expressions of IFN-γ, IL-4,IL-5, IL-10 and airway resistance of BALB / c mice. Methods The mycoplasma pneumonia infection model was builded by inhalation in BALB / c mouse; The animal model was identified by RT-PCR and HE staining method, the levels of IFN-γ, IL-4, IL-5,IL-10 in bronchoalveolar lavage fluid(BALF) were measured by ELLSA method, inspiratory resistance, expiratory resistance and pulmonary dynamic compliance were detected by AniRes2005 pulmonary function instrument. Results Compared with normal control group, the levels of IFN-γ, IL-4, IL-5, IL-10 in BALF were increased significantly after mycoplasma pneumonia infection, P<0.05; and the inspiratory resistance and expiratory resistance of mice in MP group was lower than mice in normal control group, P<0.05; pulmonary dynamic compliance of mice was lower in MP group than in normal control group. Conclusion The inspiratory resistance and expiratory resistance of mice was increased, pulmonary dynamic compliance was lower after mycoplasma pneumonia infection, and inflammation lasted long enough.更多还原