【摘要】 目的观察人乳腺癌组织中硒结合蛋白1(SBP1)和凋亡调节蛋白(Caspase-3)的表达,探讨SBP1和Caspase-3在乳腺癌发生、发展过程中的作用及其相互关系。方法用免疫组织化学ABC法检测乳腺癌中SBP1和Caspase-3的表达水平,并与癌旁相对正常组织、乳腺良性病变组织进行比较分析。结果 SBP1、Caspase-3在癌旁相对正常乳腺组织中的阳性表达率为100%、86.6%;在乳腺良性病变组织的阳性表达率为93.3%、73.3%;在乳腺癌组织的阳性表达率为82.1%、57.1%。乳腺癌组织中SBP1和Caspase-3的表达水平均明显低于癌旁相对正常组织和乳腺良性病变组织。乳腺癌组织中SBPl与Caspase-3的表达呈正相关。结论乳腺癌组织中SBP1和Caspase-3的表达下调,提示SBPl可能通过促进肿瘤细胞的凋亡来抑制肿瘤的增殖,为进一步对硒诱导SBP1治疗乳腺癌的作用研究提供基础。更多还原

【Abstract】 Objective To investigate the relationship of the expression of SBP1 and Caspase-3 in human breast cancer. Methods The human breast cancer tissues, para-carcinoma breast tissues and breast benign lesions were collected, the expression of SBP1 and Caspase-3 in tissues were detected by immunohistochemistry, and the correlation between SBP1 and Caspase-3 were assessed by statistical approach. Results The expression rate of SBP1 and Caspase-3 were 100% and 86.6% in paracarcinoma breast tissues, 93.3% and 73.3% in breast benign lesions, 82.1% and 57.1% in breast cancer tissues, respectively. Expression rate of SBP1 and Caspase-3 in breast cancer tissues was significantly lower than that in para- carcinoma breast tissues and breast benign lesions(P<0.05). SBP1 and Caspase-3 expressions were positively correlated(P<0.05). Conclusion Down-regulation of SBP1 and Caspase-3 in breast cancer tissues suggest that SBP1 may inhibit tumor growth by promoting apoptosis of tumor cells, which may provide a basis for future studies to use selenium to treat breast cancer.更多还原