【摘要】 目的探讨脊髓背根神经节自噬与大鼠术后持续性疼痛形成的关系。方法雄性成年SD大鼠,体重220~250 g,随机分为3组(n=12):空白对照组(C组)、假手术组(S组)、皮肤/肌肉切口牵拉痛(SMIR)术后持续性疼痛模型组(SMIR组)。SMIR组皮肤/肌肉切口牵拉痛模型手术,S组行皮肤切开,但不进行组织牵拉,C组不进行手术操作。各组分别于术前1 d、术后1、3、7、12 d时,测定机械缩足反应阈值(MWT)。上述各时点痛阈测试完毕后,术后第12天测试MWT结束后每组随机取4只大鼠,取手术侧L4~5背根神经节电镜下观察自噬体情况,每组随机取4只大鼠,取手术侧L4~6背根神经节,采用Western blot测定自噬标记蛋白LC3Ⅱ表达。结果与S组比较,SMIR组术后3、7、12 d MWT降低(P<0.05),手术侧脊髓背根神经节LC3Ⅱ表达上调(P<0.05)。电镜结果显示,与S组和C组相比,SMIR组手大鼠术侧背根神经节可见明显自噬结构。结论脊髓背根神经节自噬可能参与了大鼠术后持续性疼痛的形成。更多还原

【Abstract】 Objective To investigate the correlation between autophagy in dorsal root ganglia and persistent postoperative pain. Methods Male Sprague- Dawley rats,weighting 220 ~ 250 g,were randomly divided into 3 groups( n =12) : Naive control group( Group C),Sham control group( Group S) and SMIR operation group( Group SMIR). Pain behavior was assessed by mechanical paw withdrawal threshold( MWT) to von Frey filament stimulation 1 day before and 1,3,7,12 days after operation. L4 ~ 5dorsal root ganglia of SMIR rats were investigated with electro microscope. Expression of autophagy maker LC3Ⅱ was qualified by Western blotting. Results Compared with Group C and Group S, MWT was significantly reduced 3、7、12 days after SMIR surgery( P < 0. 05). LC3Ⅱ in ipilateral side of dorsal root ganglia was up- regulated following SMIR surgery. The electron microcopy showed obvious autophagy structure in SMIR rats. Conclusion Autophagy in dorsal root ganglia may participates in the pathogenesis of persistent postoperative pain.更多还原