【摘要】 为探明不同施肥模式下土壤肥际微域内微生物(细菌、真菌、放线菌)的数量分布变化,采用土柱模拟方法,研究4种施肥模式下肥际微域内细菌、真菌和放线菌的数量分布特征。结果表明,与单施化肥相比较,有机肥、化肥与有机肥配施均明显增加土壤肥际微域内细菌、真菌和放线菌的数量;增加的细菌、真菌主要分布在0~1.0cm和0~2.0cm的肥际微域中;对长期不施肥的土壤,增加的放线菌集中在0~1.0cm肥际中。不施肥时,两种供试土壤的三类微生物数量无显著差异;但施用有机肥后,长期不施肥的潮土中的细菌、真菌和放线菌的数量分别比长期施用化肥的土壤增高57%、65%和130%,说明长期施用化肥可能通过改变土壤理化生物性质,进而影响到微生物的生长繁殖。更多还原

【Abstract】 To explore the quantitative distribution variation of soil microbes under different fertilization model,the distributions of soil microbes(bacteria,fungi and actinomycetes)in fertilizer microsites in fluvo-aquic soil were studied by a simulation experiment of soil columns.The results showed that the amount of soil microbes(bacterium,fungi and actinomycetes)in fertilizer microsites of single organic manure(OM)and 70% chemical fertilizer+30% organic manure(CFOM)were significantly increased, compared with the fertilizer microsites of chemical fertilizers(CF).The amount of bacterium and fungi sharply increased in the 0~1.0cm and 0~2.0cm profiles,while the actinomycetes in long-term nofertilizer soils mainly increased in 0~1.0cm profiles.And no significant variation of microbes amount were found in two soil materials without fertilizer.However,compared with the long-term application of chemical fertilizer,the amount of bacteria,fungi and actinomycetes in no-fertilized soil,respectively, increased by 57%,65%and 130%.Therefore,the long-term chemical fertilizers may through the change of soil physical,chemical and biological properties,which affect the growth of microorganisms.更多还原