【摘要】 抽穗期(heading data,HD)和株高(plant height,PH)是水稻(Oryza sativa L.)重要的农艺性状。本研究利用金23B(Jin23B)和CR071构建了BC3F1群体及其衍生的BC3F2群体,建立的遗传连锁图谱包含140对SSR标记和5对InDel标记,较好的覆盖了水稻的12条染色体。两年共定位到了12个抽穗期相关QTLs(quantitative trait loci),8个株高相关的QTLs。其中抽穗期和株高最大效应QTL均由CR071提供,且都定位于第7染色体同一位置:抽穗期QTL qHD7 2011年LOD为33.26,解释的表型贡献率为38.2%,加性效应为10.40;2012年LOD为31.66,可以解释的表型贡献率为30.3%,加性效应为12.28;株高QTL qPH7同2011年LOD为48.72,解释的表型贡献率为44.2%,加性效应为19.97;2012年LOD为55.78,可以解释的表型贡献率为56.7%,加性效应为17.73。qHD7和qPH7都位于RM501-RM542之间,Ghd7也位于这一区间,该QTL可能就是Ghd7。抽穗期QTL qHD1-2和株高QTL qPH1-2同样都位于RM572-RM562之间,可以延迟抽穗期5 d左右和增加株高5 cm左右,qHD1-2两年内的LOD值分别为:7.58和7.03,可以解释的表型变异率分别为:6.84%和5.21%;qPH1-2两年内的LOD值分别为:4.21和4.77,可以解释的表型变异率为3.20%和3.64%,这一位点没有相关QTL定位或基因克隆的报道,位于这一区间的这两个性状也可能是由同一基因控制的。本研究通过发掘新的抽穗期和株高QTL,来增加基因的资源的多样性,为育种家利用分子标记辅助选择培育新品种提供更多的选择。更多还原

【Abstract】 Heading data(HD) and plant height(PH) are two important agronomic traits in rice(Oryza sativa L.). To analyse the HD and PH, we established BC3F1 and the derived BC3F2 backcross population of Jin 23B/CR071, to construct genetic linkage map contains 140 pairs of SSRs and 5 InDel-markers, covering 12 rice chromosomes. In two years, 12 heading data QTLs(quantitative trait loci) and 8 plant height QTLs were identified. HD and PH maximum effect QTLs are derived from the same location on chromosome 7 that contributed by parent CR071: QTL qHD7, in 2011, with a LOD score of 33.26, could explain 38.2% of the phenotypic variation and the additive effect for 10.40; In 2012, with a LOD score of 31.66, could explain 30.3% of the phenotypic variation, and the additive effect for 12.28; QTL qPH7, in 2011, with a LOD score of 48.72, could explain 44.2% of the phenotypic variat ion, and the additive effect for 19.97; In 2012, with a LOD score of 55.78, could explain 56.7% of the phenotypic variation and the additive effect for 17.73. qHD7 and qPH7 are both located between RM501-RM 542, where is located Ghd7, and it may be Ghd7. HD QTL qHD1-2 and PH QTL qPH1-2 are both located between RM572-RM562, can be delayed heading data about 5 d and adding plant height about 5 cm, qHD1-2 within two years, with a LOD score of 7.58 and 7.03, could explain 6.84% and 5.21% of the phenotypic variation; qPH1-2 within two years, with a LOD score of 4.21 and 4.77, could explain 3.20% and 3.64% of the phenotypic variation, in this position there is no relevant QTL mapping or gene reported and these two traits may also be controlled by the same gene. Then exploring new heading data and plant height QTL, increasing the diversity of genetic resources, breeders have more choices in breeding new varieties.更多还原