【摘要】 目的研究血管紧张素转化酶(ACE)基因插入或缺失(I/D)多态性与健康新生儿胰岛素敏感性的关系。方法 180例健康新生儿(孕周>33周,1分钟Apgar评分>7分)在出生时测量体重、身长,生后2~3天上午8~9时哺乳前取足跟血,测空腹血糖(FG)和空腹胰岛素(FI)水平,提取DNA进行ACE基因分型,用FI和内稳态模式评估值(HOMA)衡量胰岛素敏感性。结果 ACE基因分布符合Hardy-Weinberg平衡定律(χ2=0.188,P=0.910)。不同ACE基因型间出生体格无差异。DD基因型的HOMA对数值最高(0.21±0.45),但与ID和II基因型相比差异无显著性。DD基因型与≥1个I等位基因(即ID+II基因型)比较,DD基因型FI对数值较高(0.93±0.41比0.76±0.36,P=0.018),DD基因型的HOMA对数值较高(0.21±0.45比0.01±0.38,P=0.010)。结论在健康新生儿,D等位基因与相对差的胰岛素敏感性相关。这可能是解释远期胰岛素抵抗与D等位基因相关的线索。更多还原

【Abstract】 Objective Our goal was to investigate the relationship between angiotensin-converting enzyme gene insertion/deletion polymorphism and the insulin sensitivity in healthy newborns. Method One hundred eighty healthy newborns, all of whom had a 1-minute Apgar score of >7 and gestational age >33 weeks, were enrolled in the study. Fasting glucose and insulin levels were measured on day 2 or 3 after birth, and angiotensin-converting enzyme genotype was determined.Result The observed frequency distribution of angiotensin-converting enzyme genotypes did not deviate from that predicted by Hardy-Weinberg equilibrium in this group. There were no statistically significant differences in birth size and shape in different angiotensinconverting enzyme genotypes. Those carriers of the genotype homozygous for the deletion allele had the highest logarithmically transformed homeostasis model assessment(HOMA) compared with those who were heterozygous or homozygous for the insertion polymorphism. When compared with those with ≥1 insertion allele, those of the genotype homozygous for the deletion allele had significantly higher logarithmically transformed fasting insulin and logarithmically transformed homeostasis model assessment results. Regarding birth weight, birth length, ponderal index, and fasting glucose concentration, there were no significant differences between the genotype homozygous for the deletion allele and the genotypes heterozygous or homozygous for the insertion allele. Conclusion In this study, the deletion allele was associated with relatively impaired insulin sensitivity in healthy neonates. It may be a clue to explain the association between the deletion allele and insulin resistance in the long-term.更多还原