【摘要】 目的探讨一氧化氮(nitric oxide,NO)在大鼠软组织挤压伤后肝组织TNF-α和IL-1β基因表达中的作用。方法将大鼠随机分成对照组、挤压组、挤压后氨基胍(aminoguanidine,AG)处理组、挤压后L-精氨酸(L-arginine,L-Arg)处理组,经实验处理后检测血清中ALT、AST活性和NO浓度,并利用RT-PCR技术观察肝组织中TNF-α、IL-1β基因表达情况。结果与对照组比较,挤压组肝组织中TNF-α、IL-1βmRNA表达明显上调(P<0.05),应用L-Arg后TNF-α、IL-1βmRNA表达进一步上调(P<0.05),而应用AG后上述指标明显下降(P<0.05);与对照组比较,挤压组血清ALT、AST活性以及NO浓度明显增加(P<0.05),应用L-Arg后血清ALT、AST活性以及NO浓度进一步明显增高(P<0.05),应用AG后上述指标则明显降低(P<0.05)。结论大鼠软组织挤压伤诱导NO生成增多,内源性NO介导肝组织TNF-α和IL-1β基因表达上调。更多还原

【Abstract】 Objective To explore the effect of nitric oxide(NO) on the gene expression of hepatic TNF-α and IL-1β by crush injury of rat’s soft tissues. Methods Rats were randomly divided into sham group, crush group, crush+aminoguanidine(AG) group, and crush+L-arginine(L-Arg) group. Activities of ALT and AST as well as NO level in serum were measured. Gene expressions of TNF-α and IL-1β were detected with RT-PCR. Results Obvious increase in TNF-α and IL-1β mRNA expression was detected in the crush group compared with the sham group(P<0.05). After pretreated L-Arg, expressions of TNF-α and IL-1β mRNA were markedly increased(P<0.05). After pretreated AG, those indices obviously decreased(P<0.05). Activities of ALT and AST enhanced and NO level increased in the crush group compared with the sham group(P<0.05). Pretreatment with L-Arg or AG led to substantial increased or reduced activities of ALT and AST as well as NO levels, respectively. Conclusion Endogenous NO mediated TNF-α, IL-1β mRNA up expression in liver induced by increased production of NO after crush injury of rat’s soft tissues.更多还原