【摘要】 目的研究蛇葡萄素对人乳腺癌MCF-7细胞增殖与凋亡的影响。方法用MTT比色法检测蛇葡萄素对MCF-7细胞的增殖抑制作用,绘制量效曲线并计算IC50,流式Annexin V/PI双染法检测细胞凋亡率。结果蛇葡萄素能明显抑制MCF-7细胞增殖,IC50为19.15μg/mL;蛇葡萄素对MCF-7细胞作用6 h后,细胞凋亡率显著增加,且呈量效关系。结论蛇葡萄素能显著抑制MCF-7细胞的增殖,诱导细胞凋亡。更多还原

【Abstract】 Objective To observe the effect of ampelopsin on proliferation and apoptosis of human breast cancer MCF-7 cells. Methods The inhibitory effect of ampelopsin on proliferation of MCF-7 cells was detected by MTT colorimetricassay, the dose-response curve was drawn, the IC50was calculated, and the apoptotic rate was detected by Annexin V / PI double-staining method. Results Ampelopsin inhibited the proliferation of MCF-7 cells, the IC50was 19.15 μg / mL. After the MCF-7 cells were treated with ampelopsin for 6 hours, the apoptotic rate was increased significantly, and does-effect relationship existed in the both two sides. Conclusion Ampelopsin can inhibit the proliferation of MCF-7 cells significantly, and induce the apoptosis.更多还原