【摘要】 目的研究益肠平对溃疡性结肠炎小鼠结肠通透性增高的预防作用并初步探讨其机制。方法采用2.5%葡聚糖硫酸钠定量灌胃诱导小鼠溃疡性结肠炎(UC)。小鼠随机分为正常对照组、模型对照组、柳氮磺吡啶组及益肠平高、中、低剂量组,测定各组小鼠结肠损伤大体评分(MACN)、结肠通透性、结肠组织丙二醛(MDA)、超氧化歧化酶(SOD)及诱导型一氧化氮合酶(iNOS)。分析结肠通透性与上述指标的相关性。结果与模型对照组比较,益肠平能降低UC小鼠MACN、结肠组织MDA及iNOS,升高结肠组织SOD,降低结肠通透性,并呈剂量依赖性。UC小鼠结肠通透性改变与MACN、MDA、SOD、iNOS密切相关。结论益肠平对UC小鼠结肠通透性增高有良好的预防作用,其机制可能与其降低UC小鼠结肠自由基及结肠通透性有关。更多还原

【Abstract】 Objective To investigate the preventive effect of " Yi Chang Ping" on the colonic permeability in ulcerative colitis in mice and the mechanism was primarily discussed. Methods Colitis was induced by intragastric administration of 2.5% dextran sulfate sodium. Mice were divided into six groups randomly:normal control group,model control group,sulfasalazine control group,and large,medium and low dosage treated groups. The macroscopic assessment of colonic damage(MACN),colonic permeability,activities of malondialdehyde,superoxide dismutase in colonic tissues and inducible nitric oxide synthase were performed. The correlations of these indexes were analysised. Results Compared with the model contronl group,MACN,MDA,iNOS and colonic permeablilty in colonic tissues was significant decreased and SOD in colonic tissues significant increased in"Yi Chang Ping"treated groups. The improvements of above-mentioned parameters were dose dependent. There were good correlations between the colonic permeability and MACN,MDA,iNOS,SOD. Conclusion "Yi Chang Ping"has good preventive effect on colonic permeability of the UC mice. Its mechanism may be involved in the reduction of oxygen free radical and colonic permeabilty.更多还原