In order to analysis and determination of sucrose in sugarcane reaping in different times. In the study,sampling water was used as solvent. HPLC is uesd,the chromatographic separation was achieved on an Waters Sugar PakⅠ column( 6. 5 mm × 300 mm,10. 0 μm),which was held at a constant temperature of 90 ℃,RID temperature was set as 35 ℃,ultra-high purity water as moving phase,at a flow rate of 1. 0 mL·min- 1. The result shows average spike recoveries for 5 replicate determinations of three saccharides were fo...