【摘要】 以多效唑诱导中国水仙的抑制差减杂交文库(SSH)获得的cDNA片段为基础,采用RACE及RT-PCR技术,分离克隆到1个ATP合成酶CF0B亚基基因NtATPF。此基因包含有1个555bp完整阅读框架(ORF),编码184个氨基酸,具有1个跨膜区域(27~49),分子量为20.809 5kD,理论等电点为5.69。聚类分析表明:其氨基酸序列与葱的叶绿体ATP合成酶CF0B亚基蛋白氨基酸序列亲缘关系最近。更多还原

【Abstract】 Based on a fragment cDNA from suppression subtractive hybridization(SSH)of Narcissus tazetta var.chinensis induced by paclobutrazol,an ATP Synthase CF0 subunit gene NtATPF was separated and cloned by RACE and RT-PCR.The full length of NtATPFcDNA was 905 bp with a 555 bp ORF,which encoded a protein of184 amino acids and had a transmembrance domain(27-49)with a predicted molecular mass of 20.809 5kD and theoretical isoelectric point of 5.69.Phylogenetic analysis showed that NtATPF had close relationship to ATP Synthase CF0 subunit in Allium fistulosum(AEX93661.1).更多还原