【摘要】 简单序列重复(SSR)是共显性遗传标记,可有效地反映个体遗传信息和亲缘关系,在杂交检测、物种鉴定及种群遗传学研究等方面应用广泛.作为重要的经济植物,西藏沙棘是青藏高原植物生态适应与进化研究的极佳材料,但目前还缺乏对其种质资源和遗传结构的了解.利用5’锚定PCR方法和磁珠富集法开发西藏沙棘的SSR引物.共得到13个种内多态性的SSR位点,分别有2~4个等位基因,观测杂合度(HO)从0.00到0.74不等,预期杂合度(HE)从0.04到0.54不等.两种方法所得到的引物可用于西藏沙棘的种质资源鉴定、遗传结构研究等多个方面.更多还原

【Abstract】 As a kind of co-dominant genetic markers,microsatellites can effectively reflect individual genetic information and genetic relationships.It has been widely used in detection of hybrids,species identification and population genetics.Hippophae tibetanais an ideal material for studying adaptive evolution of important ecological species from the Qinghai-Tibetan Plateau.However,little is known about its germplasm resources and genetic structure.Here,microsatellite primers for Hippophae tibetana using 5’anchored PCR methods and magnetic beads hybridization were developed.Total 13 polymorphic loci were obtained,each having two to five alleles.Observed heterozygosity(HO)at these loci ranged from 0.00 to 0.74,and the expected heterozygosity(HE)from0.04 to 0.54.These primers can be directly applied to the germplasm resources identification,genetic structure study of this species and other aspects.更多还原