【摘要】 为探讨乙二醛(GO)和甲基乙二醛(MGO)对上皮细胞的细胞毒性及其机制,分别以MTT法检测两种活性羰基化合物(RCS)对ECV304细胞活力的影响,评价其细胞毒性;DCFH-DA探针法检测氧化应激;用罗丹明123检测线粒体膜电位及线粒体形态变化。结果表明,GO和MGO对ECV304细胞的IC50分别为3.6mmol/L±0.1mmol/L和1.3mmol/L±0.1mmol/L;氧化应激水平小幅度增高,线粒体膜电位随发生改变;线粒体形态发生改变。GO和MGO的细胞毒性与氧化应激水平增加相关性较低而与线粒体损伤相关。更多还原

【Abstract】 To explore the cytotoxicity and its mechanism of GO and MGO on epithelial cells,the cytotoxicity of two reactive carbonyl species were measured by MTT reduction assay.Oxidative stress was detected by a non-specific ROS probe(DCFH-DA),and the MMP and mitochondrial morphological changes were detected by using rhodamine 123.The IC50 of GO and MGO on ECV304 cells respectively are 3.6mmol/L±0.1mmol/L and 1.3mmol/L±0.1mmol/L.The level of oxidative stress had a slight increase while the mitochondrial membrane potential was alterative,and mitochondrial swelling occurred.In conclusion,the cytotoxicities of GO and MGO were mainly due to the mitochondrial dysfunction,and showed weak correlation with oxidative stress.更多还原