【摘要】 为研制沙门菌鞭毛蛋白佐剂禽流感疫苗,以盐酸裂解法制备和纯化鸡肠炎沙门菌鞭毛蛋白,将其作为佐剂,包裹灭活的禽流感病毒(H9N2亚型),制备禽流感灭活疫苗并进行动物试验。结果表明,该疫苗分别经滴鼻和肌肉注射接种后,虽然没有明显提高免疫鸡的血凝抑制(HI)抗体水平,但显著提高了血清中和呼吸道黏膜分泌液中抗禽流感病毒特异性IgA水平;攻毒试验结果表明,经滴鼻接种后,单独使用灭活的AIV没有产生免疫保护作用(0/15),而鞭毛蛋白佐剂灭活苗免疫组保护率达53.3%(8/15);经肌肉注射接种后,单独免疫灭活的AIV组的保护率为30%(3/10),而鞭毛蛋白佐剂灭活苗免疫组保护率达60%(6/10)。表明沙门菌鞭毛蛋白能够有效促进禽流感灭活疫苗的免疫保护效果。更多还原

【Abstract】 To test whether Salmonellaflagellin can be as potent adjuvant in preparation of novel avian influenza vaccine,in this study,flagellins of Salmonella gallinarum were prepared and purified by using hydrochloric acid decomposition method.The vaccine developed by the complex of flagellins and inactivated H9N2 subtype avian influenza virus was evaluated by animal experiments.The results showed that this adjuvented vaccine induced significant IgA level from the mucous of the respiratory tract and plasma after being inoculated into chickens via either intranasal or intramuscular route,compared with inactivated avian influenza virusalone,although Salmonellaflagellins didn’t improve hemagglutinin inhibition(HI)antibody in vaccinated chickens.The challenge experiments indicated that intranasal immunization with Salmonellaflagellin adjuvanted vaccine induced 53.3%of protection,but no protection(0/15)was achieved from the same inoculation route with inactived AIV alone.However,intramuscular immunization with combination of flagellins and inactivated AIV exhibited 60% of protection,which was greater than that with inactivated AIV alone(30%).It demonstrated that Salmonellaflagellin could enhance protection efficacy of inactivated AI vaccine.更多还原