【摘要】 探讨了大鲵(Andrias davidianus)胚胎发育过程中受精率的评判标准和计算受精率的最佳时间。对大鲵胚胎发育观察表明:发育到二细胞的胚胎均能发育到神经胚期,因此可以将二细胞胚胎期作为受精的标志;孵化温度在17~20℃时,温度对大鲵的孵化率没有影响;胚胎发育经过24 h后,有90%~95%的受精卵可发育到二细胞期。因此可以以受精后24 h的二细胞分裂数作为计算大鲵胚胎受精率的基础数据。研究结果有利于对大鲵人工繁育效果提前判断和对孵化条件及时调控处理。更多还原

【Abstract】 The relational fertilization tests of Chinese Andrias davidianus were carried out in order to analyze and evaluate the artificial insemination in early stage. During continuous observation of embryo development at early stages,the results proved that embryo at the two cell stage had the ability to develop dynamically to gastrula stage and neurula stage. Comparative analysis the time that fertility rate reached 90% and 95% revealed that 24 h after fertilized can be used as the optimal time. Combined the results,the embryo development at two cell stage and 24 h post-fertilization can serve as the judgment standard of fertility rate. The results served the important guidance for captive breeding in Chinese giant salamander.更多还原