【摘要】 为研究AtTIP5;1基因的功能,以非洲紫罗兰叶片为受体材料,进行AtTIP5;1基因遗传转化条件的研究,并对转基因植株在高浓度硼酸胁迫下的表现进行分析,以明确利用AtTIP5;1基因提高非洲紫罗兰抗逆性的可行性。结果表明:(1)以非洲紫罗兰叶片为受体的最适转化条件为:叶片预培养2d,农杆菌共培养2d,共培养时添加100μmol/L乙酰丁香酮;最适潮霉素筛选浓度为40mg/L。(2)转化植株经PCR和RT-PCR检测显示,AtTIP5;1基因成功转入了非洲紫罗兰,获得了17个稳定的转基因植株。(3)对转基因植株和对照植株进行3mmol/L硼酸胁迫处理结果表明,AtTIP5;1基因表达受高浓度硼酸诱导,且AtTIP5;1基因表达可以减缓植株受高浓度硼酸胁迫导致的组织褐化、叶片卷曲变形症状;转AtTIP5;1基因植株干物质率、可溶性糖、可溶性蛋白质含量下降,而POD、SOD活性上升,证明转AtTIP5;1基因的非洲紫罗兰植株提高了对高浓度硼酸的耐受能力。研究结果为深入探讨AtTIP5;1基因的功能以及非洲紫罗兰基因工程育种奠定了基础。更多还原

【Abstract】 In order to study the function of AtTIP5;1 gene,we employed Saintpaulia ionanthaleaves as receptor materials and optimized the genetic transformation conditions.The transgenic plants were exposed to high concentration of boric acid and the resistant ability was evaluated.The results showed:(1)The receptor leaves pre-cultured for 2d,infected for 15 min,co-cultured of 2d with 100μmol/L AS,and selected with 40 mg/L hygromycin were the optimum transformation conditions.(2)After PCR and RT-PCR screening,17 transformed plants containing AtTIP5;1 transgene were obtained.(3)Transformed and control plants were treated by high concentration boric acid(3 mmol/L)and physiological indexes of these plants were evaluated.The results indicated that the AtTIP5;1 expression could be induced by boron.Transgenic plants showed a lower degree of plant tissue browning,leaves curling,and a higher rate of dry matter,soluble sugar and soluble protein content.Meanwhile,the increase of POD and SOD activities were weaken.Our results demonstrate that AtTIP5;1 trasformation can improve the tolerance of S.ionanthato the high boron stress.Our study is helpful for further investigation on AtTIP5;1 gene function and gene engineering in breeding of S.ionantha.更多还原