【摘要】 利用苹果栽培品种‘红富士’和新疆野苹果优系‘红肉苹果’杂交的110个F1株系为作图群体,构建了苹果的分子遗传图谱,采用区间作图法对苹果9个叶片相关性状(叶片长度、叶片宽度、叶片厚度、叶柄长度、叶片面积、总叶绿素含量、叶绿素a含量、叶绿素b含量和类胡萝卜素含量)进行了QTL定位分析。结果显示:从110个F1株系中共检测到20个控制叶片相关性状的QTL位点,分布在第1、2、3、4、5、7、8、10、11、12、16、17连锁群上;各QTL位点的LOD值在2.58~3.55之间,其中主效QTL位点2个(LOD≥3.5),可解释11.63%~16.36%的表型变异。获得紧密连锁的特异标记(CH05d11-435m、CH04c06-201m)为进一步进行QTL精细定位提供了参考。更多还原

【Abstract】 In this study,we mapped and characterized quantitative trait loci(QTL)affecting apple leaf traits.A total of 110F1individuals obtained by crossing between a good quality cultivar‘Red Fuji’of Malus domestica and an accession‘Hongrou Apple’of Malus sieversii were used to construct a 280-markers map in apple.By using interval mapping,3QTLs for leaf length,1QTLs for leaf width,3QTLs for leaf thickness,5QTLs for leafstalk length,1QTL for leaf area,1QTL for chlorophyl a content,2QTLs for chlorophyl b content,2QTLs for chlorophyl content,2QTLs for carotenoid content were identified.These QTLs were mapped on chromosome 1,2,3,4,5,7,8,10,11,12,16and 17,respectively.The phenotypic variations explained by each QTL ranged from 11.63%to 16.36%,and their LOD values varied from 2.58to 3.55,of which two QTLs were major gene(LOD values≥3.5).The tight linkage markers(CH05d11-435m and CH04c06-201m)can be used in fine mapping of leaf length and chlorophyl b content in apple.更多还原