Fabrication of a Bi-layer Tubular Scaffold Consisted of a Dense Nanofibrous Inner Layer and a Porous Nanoyarn Outer Layer for Vascular Tissue Engineering

【摘要】 Recent years,it has attracted more attentions to increase the porosity and pore size of nanofibrous scaffolds to provide the microenvironment for the cells to grow into the small-diameter vascular grafts.In this study,a novel bi-layer tubular scaffold with an inner layer and an outer layer was fabricated.The inner layer was random collagen / poly( L-lactide-co-caprolactone) [P( LLACL) ] nanofibrous mat fabricated by conventional electrospinning and the outer layer was aligned collagen / P( LLA-CL) nanoyarns prepared by a dynamic liquid electrospinning method.Fourier transform infrared spectroscopy( FTIR) was used to characterize the chemical structure.Scanning electron microscopy( SEM) was employed to observe the morphology of the layers and the crosssectioned bi-layer tubular scaffold.A liquid displacement method was employed to measure the porosities of the inner and outer layers.Stress-strain curves were obtained to evaluate the mechanical properties of the two different layers and the bi-layer membrane.The diameters of the nanofibers and the nanoyarns were( 480 ± 197)nm and( 19.66 ± 4.05) μm,respectively.The outer layer had a significantly higher porosity and a larger pore size than those of the inner layer.Furthermore,the bi-layer membrane showed a good mechanical property which was suitable as small-diameter vascular graft.The results indicated that the bi-layer tubular scaffold had a great potential application in small vascular tissue engineering.更多还原

【Abstract】 Recent years,it has attracted more attentions to increase the porosity and pore size of nanofibrous scaffolds to provide the microenvironment for the cells to grow into the small-diameter vascular grafts.In this study,a novel bi-layer tubular scaffold with an inner layer and an outer layer was fabricated.The inner layer was random collagen / poly( L-lactide-co-caprolactone) [P( LLACL) ] nanofibrous mat fabricated by conventional electrospinning and the outer layer was aligned collagen / P( LLA-CL) nanoyarns prepared by a dynamic liquid electrospinning method.Fourier transform infrared spectroscopy( FTIR) was used to characterize the chemical structure.Scanning electron microscopy( SEM) was employed to observe the morphology of the layers and the crosssectioned bi-layer tubular scaffold.A liquid displacement method was employed to measure the porosities of the inner and outer layers.Stress-strain curves were obtained to evaluate the mechanical properties of the two different layers and the bi-layer membrane.The diameters of the nanofibers and the nanoyarns were( 480 ± 197)nm and( 19.66 ± 4.05) μm,respectively.The outer layer had a significantly higher porosity and a larger pore size than those of the inner layer.Furthermore,the bi-layer membrane showed a good mechanical property which was suitable as small-diameter vascular graft.The results indicated that the bi-layer tubular scaffold had a great potential application in small vascular tissue engineering.更多还原