【摘要】 目的研究香附对川芎中阿魏酸(ferulic acid,FA)在正常及偏头痛模型大鼠体内的药代动力学影响。方法用硝酸甘油(10mg/kg)制作偏头痛大鼠模型,SD大鼠分为正常川芎组、正常川芎-香附组、模型川芎组、模型川芎-香附组,灌胃给药,采用高效液相色谱法(HPLC)检测血药浓度,流动相为0.2%甲酸水(A)-甲醇(B),梯度洗脱,检测波长为322nm,检测给药后0~8h内大鼠血浆中FA的浓度,DAS 2.0软件计算药代动力学参数。结果 HPLC法的方法学考察均符合测定要求。正常组中川芎配伍香附后FA的吸收半衰期t1/2Ka缩短(P<0.05),达峰时间Tmax提前(P<0.05),但其他药代动力学参数与配伍前比较差异无统计学意义。模型组中川芎配伍香附后FA的达峰浓度Cmax、药时曲线下面积AUC(0-t)、分布半衰期t1/2α、表观分布容积增大(P<0.01或P<0.05),Tmax提前(P<0.05),清除率降低(P<0.01)。结论在正常大鼠体内香附加快了FA的吸收,而在偏头痛模型大鼠体内香附减慢FA在体内的分布和代谢,延长药物在体内的作用时间,从而提高了FA的生物利用度。更多还原

【Abstract】 Objective To investigate the effect of rhizoma cyperi on pharmacokinetics of ferulic acid in ligusticum wallichii in normal and nitroglycerin-induced migraine model rats.Methods The rat migraine model was created by nitroglycerin(10 mg/kg).SD rats were divided into normal rhizoma ligustici wallichii group,normal rhizoma ligustici wallichii and rhizoma cyperi group,model rhizoma ligustici wallichii group,and model rhizoma ligustici wallichii and rhizoma cyperi group.We used RP-HPLC(0.2%formic acid water[A]-methanol[B],gradient elution 322nm)to detect the concentration of ferulic acid in rat plasma at 8hafter oral administration.DAS 2.0software was used to calculate the pharmacokinetic parameters.Results Methodology inspection conformed that HPLC met the requirements for determination.Compared with normal rhizoma ligustici wallichii group,the absorption half time(t1/2Ka)of ferulic acid was significantly reduced in normal rhizoma ligustici wallichii and rhizoma cyperi group(P<0.05),and the peak time(Tmax)was significantly shortened(P<0.05),with no differences found for other parameters.Compared with model rhizoma ligustici wallichii group,model rhizoma ligustici wallichii and rhizoma cyperi group had significantly increased maximum plasma concentration(Cmax),area under curve(AUC),distribution half-life time(t1/2α),and apparent volume of distribution of ferulic acid(P<0.05 or P<0.01),significantly shortened Tmax(P<0.05),and significantly decreased the absorption rate and the clearance speed(P<0.01).Conclusion Rhizoma cyperi enhances the absorption of ferulic acid in normal rats,while in migraine model rats it reduces the distribution and metabolism of ferulic acid and prolongs functioning time,subsequently promoting the bioavailability of ferulic acid.更多还原