【摘要】 以马鹿(Cervus elaphus)为试验动物,采用上浮法诱导马鹿精子体外获能,以钙离子载体(A23187)诱导马鹿精子顶体反应,进而摸索钙离子载体(A23187)诱发其顶体反应的适宜体系。结果表明:0.01~100.00μmol/L的A23187可提高获能马鹿精子的顶体反应率;其中适宜的A23187浓度为0.10μmol/L,反应的时间为5 min,顶体反应率为(74.00±2.62)%。A23187能促进获能马鹿精子的顶体反应,但不能显著(P>0.05)促进未获能马鹿精子的顶体反应。更多还原

【Abstract】 Wapiti( Cervus elaphus) sperm was capacitated in vitro by swimming-up and the acrosome reaction( AR) was induced by Calcium ionophore A23187 to choose an optimal A23187 system during AR. A23187 of 0.01- 100 μmol / L can enhance the ratio of AR on the capacitated sperm and 0.1 μmol / L A23187 can obtain the highest ratio of AR( 74.00%±2.62%) for 5-min treatment time. AR of capacitated sperm can,but the uncapacitated sperm cannot,be induced by A23187in wapiti.更多还原