【摘要】 根据已公布液泡膜H+-PPase基因家族同源序列保守区设计简并引物,克隆出海滨雀稗中PvVP1基因的中间序列,然后用快速扩增cDNA末端(rapid amplification of cDNA end,RACE)方法,从海滨雀稗中克隆到PvVP15′cDNA序列。该序列ORF长1605bp,编码535个氨基酸,其编码序列、氨基酸序列与玉米相应基因的同源性分别为93%和99%。更多还原

【Abstract】 A pair of degenerated primers were designed based on previously cloned homologous cDNA segments.The middle segment of PvVP1 was cloned,then the PvVP1 5’cDNA sequence also obtained using 5’RACE.The open reading frame(ORF)of this sequence was 1605 bp,encoding 534 amino acids.The codon and amino acid sequences of the 5’ends of the PvVP1 gene shared 93%and 99%similarity,respectively,with those of Zea mays.更多还原