【摘要】 为寻找高效纤维素降解微生物用于蚕沙堆肥的腐熟发酵,以羧甲基纤维素钠为碳源,从泰山林地腐殖土中分离纤维素降解菌,结合纤维素刚果红水解圈测定、滤纸条降解试验和纤维素酶活力(滤纸酶活力)测定,从中筛选出一株高效纤维素降解真菌,命名为C2。经形态学鉴定和18S rDNA序列分析,鉴定该菌株为草酸青霉(Penicillium oxalicum)。该菌株有较强的滤纸条降解能力,5 d可将滤纸条降解成糊状,产纤维素酶活力高,摇瓶发酵3 d后滤纸纤维素酶活力达到132.26 U/mL,具有实用价值。更多还原

【Abstract】 In order to look for efficient cellulose degrading microorganisms and use it in composting silkworm excrement, we adopted CMC-Na as the carbon source and isolated cellulose degrading strains from woodland humus soil of Taishan Mountain. As a result,one fungal strain with high cellulose degrading efficiency was obtained and named C2 based on Congo red cellulose hydrolytic circle measurement,filter paper degradation test and cellulase(filter paper enzyme)activity assay. Morphological observation and 18S rDNA sequence analysis revealed that this strain belonged to Penicillium oxalicum. C2 strain possessed high filter paper degrading ability and could degrade the filter paper into paste within 5 days. C2 strain owns high cellulase activity. After being cultured for 3 days on a shaking bed,filter paper cellulose produced by this strain could reach 132. 26 U/ mL,being of high practical values.更多还原