【摘要】 目的探讨老年创伤患者外周血中髓源抑制细胞(MDSCs)比例变化及其临床意义。方法收集26例青年创伤患者(18~30岁)和21例老年创伤患者(>60岁),以CD14-/CD11b+/CD33+作为MDSCs标志物,流式细胞术(FCM)检测MDSCs比例,酶联免疫吸附试验(ELISA)检测血清白介素-10、转化生长因子-β和C反应蛋白水平,分析MDSCs比例的变化情况及其临床意义。结果伤后1d,老年组和青年组创伤患者外周血MDSCs比例无显著差异(P>0.05)。伤后3d和7d,老年组创伤患者外周血MDSCs比例均高于青年组,差异有显著意义(P<0.01)。MDSCs比例和血清转化生长因子-β、白介素-10和C反应蛋白水平均有相关性。结论老年创伤患者外周血MDSCs比例较青年组显著增高,可能是老年创伤患者更容易发生免疫抑制的重要机制。更多还原

【Abstract】 Objective To explore the variation and significance of CD14-/ CD11b+/ CD33+myeloid derived suppressor cells( MDSCs) in elderly patients with multiple injuries. Methods Totally 26 young patients( age 18-30 years) and 21 elderly patients( age > 60 years) with multiple injury were enrolled in this study. CD14-/CD11b+/ CD33+MDSCs in peripheral blood were determined by flow cytometry( FCM) at 1,3 and 7days after trauma. Serum transforming growth factor-β( TGF-β),interleukin-10 and C-reactive protein were determined by ELISA. Results The percentages of CD14-/ CD11b+/ CD33+MDSCs in peripheral blood of elderly patients at 3 and 7days after trauma were significantly higher than those of young patients( P < 0. 01),whereas did not differ significantly at 1 day after trauma between the two groups( P > 0. 05). The percentages of CD14-/ CD11b+/ CD33+MDSCs in peripheral blood of elderly patients with multiple injury were closely correlated with serum TGF-β,interleukin-10 and C-reactive protein level( P < 0. 05). Conclusion The percentages of CD14-/ CD11b+/ CD33+MDSCs in peripheral blood were significantly increased in elderly patients with multiple injuries. Our results indicated that accumulation of CD14-/ CD11b+/ CD33+MDSCs may be an important mechanism of aging-related immune dysfunction in elderly patients with multiple injuries.更多还原